Beneficial effects of Ginkgo biloba extract on insulin signaling cascade, dyslipidemia, and body adiposity of diet-induced obese rats

Beneficial effects of Ginkgo biloba extract on insulin signaling cascade, dyslipidemia, and body adiposity of diet-induced obese rats

Author Banin, Renata Mancini Autor UNIFESP Google Scholar
Hirata, Bruna Kelly Sousa Autor UNIFESP Google Scholar
Andrade, Iracema Senna de Autor UNIFESP Google Scholar
Zemdegs, Juliane Costa Silva Autor UNIFESP Google Scholar
Clemente, Ana Paula Grotti Autor UNIFESP Google Scholar
Dornellas, Ana Paula Segantine Autor UNIFESP Google Scholar
Boldarine, Valter Tadeu Autor UNIFESP Google Scholar
Estadella, Debora Autor UNIFESP Google Scholar
Albuquerque, Kelse Tibau de Autor UNIFESP Google Scholar
Oyama, Lila Missae Autor UNIFESP Google Scholar
Ribeiro, Eliane Beraldi Autor UNIFESP Google Scholar
Telles, Monica Marques Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Alagoas Faculdade de Nutrição
Universidade Federal do Rio de Janeiro Curso de Nutrição
Abstract Ginkgo biloba extract (GbE) has been indicated as an efficient medicine for the treatment of diabetes mellitus type 2. It remains unclear if its effects are due to an improvement of the insulin signaling cascade, especially in obese subjects. The aim of the present study was to evaluate the effect of GbE on insulin tolerance, food intake, body adiposity, lipid profile, fasting insulin, and muscle levels of insulin receptor substrate 1 (IRS-1), protein tyrosine phosphatase 1B (PTP-1B), and protein kinase B (Akt), as well as Akt phosphorylation, in diet-induced obese rats. Rats were fed with a high-fat diet (HFD) or a normal fat diet (NFD) for 8 weeks. After that, the HFD group was divided into two groups: rats gavaged with a saline vehicle (HFD+V), and rats gavaged with 500 mg/kg of GbE diluted in the saline vehicle (HFD+Gb). NFD rats were gavaged with the saline vehicle only. At the end of the treatment, the rats were anesthetized, insulin was injected into the portal vein, and after 90s, the gastrocnemius muscle was removed. The quantification of IRS-1, Akt, and Akt phosphorylation was performed using Western blotting. Serum levels of fasting insulin and glucose, triacylglycerols and total cholesterol, and LDL and HDL fractions were measured. An insulin tolerance test was also performed. Ingestion of a hyperlipidic diet promoted loss of insulin sensitivity and also resulted in a significant increase in body adiposity, plasma triacylglycerol, and glucose levels. In addition, GbE treatment significantly reduced food intake and body adiposity while it protected against hyperglycemia and dyslipidemia in diet-induced obesity rats. It also enhanced insulin sensitivity in comparison to HFD+V rats, while it restored insulin-induced Akt phosphorylation, increased IRS-1, and reduced PTP-1B levels in gastrocnemius muscle. The present findings suggest that G. biloba might be efficient in preventing and treating obesity-induced insulin signaling impairment.
Keywords Ginkgo biloba extract
Obesity
Insulin tolerance
Body adiposity
Akt phosphorylation
Rats
Language English
Date 2014-09-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 47, n. 9, p. 780-788, 2014.
ISSN 0100-879X (Sherpa/Romeo)
Publisher Associação Brasileira de Divulgação Científica
Extent 780-788
Origin http://dx.doi.org/10.1590/1414-431X20142983
Access rights Open access Open Access
Type Article
SciELO ID S0100-879X2014000900780 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/8549

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