Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin

Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin

Author Raymundo, Erica Calcagno Google Scholar
Hochman, Bernardo Autor UNIFESP Google Scholar
Nishioka, Michele Akemi Autor UNIFESP Google Scholar
Freitas, Jose Octavio Gonçalves de Autor UNIFESP Google Scholar
Maximino, Jéssica Ruivo Google Scholar
Chadi, Gerson Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract PURPOSE:To investigate the subcutaneous injection of carbon dioxide (CO2) on neuropeptides Calcitonin Gene-Related Peptide (CGRP) and Substance P (SP) secretion in rat skin. METHODS:Fifty-six Wistar-EPM rats were distributed in two groups: one for CGRP analysis, the other for SP analysis. Each group was subdivided into four subgroups: control (Cont), control with needle (ContNd), CO2 injection (CO2Inj) and atmospheric air injection (AirInj) - with seven animals each. Sample analyses of partial skin were conducted by Western Blotting (WB). RESULTS:In SP group, there was a decrease in the amount of neuropeptides in subgroups CO2Inj and AirInj. Similarly, in CGRP group, there was a decrease in the amount of pro-CGRP neuropeptides (15 kDa) in subgroups CO2Inj and AirInj; Nevertheless, there was no decrease in the amount of CGRP (5 kDa) in any subgroups. CONCLUSION:Subcutaneous injection of CO2 and atmospheric air decreased the amount of Substance P and pro-Calcitonin Gene-Related Peptide (15 kDa) neuropeptides in rat skin.
Keywords Carbon Dioxide
Substance P
Calcitonin Gene-Related Peptide
Neurogenic Inflammation
Skin
Rats
Language English
Date 2014-04-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 4, p. 224-230, 2014.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 224-230
Origin http://dx.doi.org/10.1590/S0102-86502014000400002
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000334997000002
SciELO ID S0102-86502014000400224 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/8346

Show full item record




File

Name: S0102-86502014000400224.pdf
Size: 820.5Kb
Format: PDF
Description:
Open file

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account