Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury

Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury

Author Ikejiri, Adauto Tsutomu Google Scholar
Somaio Neto, Frederico Google Scholar
Chaves, Jose Carlos Google Scholar
Bertoletto, Paulo Roberto Google Scholar
Teruya, Roberto Google Scholar
Bertoletto, Eduardo Rodrigues Google Scholar
Taha, Murched Omar Autor UNIFESP Google Scholar
Fagundes, Djalma José Autor UNIFESP Google Scholar
Institution Federal University of Grande Dourados Medical School
UFGD Medical School
Federal University of Mato Grosso do Sul Surgery Department
Universitary Center Sao Camilo
Universidade Federal de São Paulo (UNIFESP)
Abstract PURPOSE:To determine the gene expression profile associated with oxidative stress and antioxidant defense in the lung tissue of mice subjected to intestinal ischemia and reperfusion.METHODS:Twelve male, inbred mice (C57BL/6) were randomly assigned to one of two groups. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes; the ischemia/reperfusion group (IRG) was subjected to anesthesia, laparotomy, and ischemia of the small intestine for 60 minutes and to 60 minutes of reperfusion. A pool of six mice from each group was subjected to a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to analyze the oxidative stress and antioxidant defense genes. All genes that were up-regulated or down-regulated greater than three-fold, based on the algorithm [2
Keywords Gene Expression
Oxidative Stress
Lung
Reperfusion Injury
Mice
Language English
Date 2014-03-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 3, p. 186-192, 2014.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 186-192
Origin http://www.scielo.br/scielo.php?pid=S0102-86502014000300186&script=sci_arttext&tlng=pt
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000333047100007
SciELO ID S0102-86502014000300186 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/8265

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