The effect of simvastatin on the regeneration of surgical cavities in the femurs of rabbits

The effect of simvastatin on the regeneration of surgical cavities in the femurs of rabbits

Author Rosselli, Jose Eduardo Gomes Cardim Google Scholar
Martins, Dulce Maria Fonseca Soares Autor UNIFESP Google Scholar
Martins, Jose Luiz Autor UNIFESP Google Scholar
Oliveira, Claudia Regina Gomes Cardim Mendes de Google Scholar
Fagundes, Djalma José Autor UNIFESP Google Scholar
Taha, Murched Omar Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract PURPOSE:To evaluate the effect of a local application of simvastatin gel in repairing bone defects in the femurs of rabbits.METHODS:Two standard surgical cavities were created in the femoral epiphysis of 18 rabbits. In the simvastatin group (SG), the cavities were filled with a collagen sponge soaked in 0.5 ml of a simvastatin (1 mg) gel, and the cavities were covered with a biological membrane. The bone cavities in the second group (control group) were filled with a blood clot and covered with a biological membrane. On the 7th, 21st and 42nd days, six animals in each group were euthanized, and the femurs were subject to histological evaluation (vascularity, fibrosis, reactive bone formation, osteoblasts, and osteoclasts) and immunohistochemical (anti-VEGF and anti-osteocalcin) analysis. The results were analyzed using a Wilcoxon test (p<0.05).RESULTS:There were significant differences between the two groups: the SG had greater scores in comparison with the CG in terms of the degree of vascularity on the 7th and the 21st days, fibrosis on the 21st day, bone formation reaction on the 21st and the 42nd days and the number of osteoblasts and osteoclasts on the 42nd day. The immunohistochemical expression was also greater for osteocalcin and VEGF on the 7th, 21st and 42nd days.CONCLUSION:Surgical defects created in rabbit femurs were treated locally with simvastatin gel to stimulate bone repair, which promoted an ameliorative effect in the morphological and immunohistochemical markers of bone regeneration.
Keywords Simvastatin
Bone Regeneration
Bone Morphogenetic Proteins
Rabbits
Language English
Date 2014-02-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 2, p. 87-92, 2014.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 87-92
Origin http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000200087
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000333046900003
SciELO ID S0102-86502014000200087 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/8234

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