Sentinel lymph node biopsy in rats. Comparison between paraffin and frozen section analysis

Show simple item record Santos Filho, Ivan Dunshee de Abranches Oliveira [UNIFESP] Enokihara, Mílvia Maria Simões e Silva [UNIFESP] Shie, Janaina Namba [UNIFESP] Oliveira, Andrea Fernandes de [UNIFESP] Santos, Ivan Dunshee de Abranches Oliveira [UNIFESP] Ferreira, Lydia Masako [UNIFESP] 2015-06-14T13:46:53Z 2015-06-14T13:46:53Z 2014-01-01
dc.identifier.citation Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 57-61, 2014.
dc.identifier.issn 0102-8650
dc.description.abstract PURPOSE:To simulate a lymph node metastasis in an animal model using activated carbon, assess their identification in frozen section analysis and compare with histopathological examination in paraffin.METHODS:Thirty two adult female rats were used. They received the carbon injection on its hind legs. Half of the rats was sacrificed on day one, and the other half after 21 days. Thus, 64 lymph nodes were dissected and split longitudinally. One half of the lymph node was sent immediately to frozen section analysis. The other half was fixed in 10% formaldehyde to be cut in paraffin. Slides were divided into quadrants and classified by the presence of carbon in these four quadrants_ They were also classified by the carbon staining intensity.RESULTS:Comparing the slides obtained in the first day and 21 days, there was a tendency of carbon to spread over time, but without statistical significance. The intensity did not alter over time.CONCLUSION:There was no concordance between the two methods of pathological analysis, however the actived carbon was seen in all lymph nodes. en
dc.format.extent 57-61
dc.language.iso eng
dc.publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartof Acta Cirurgica Brasileira
dc.rights Acesso aberto
dc.subject Lymph node biopsy en
dc.subject Frozen sections en
dc.subject Paraffin embedding en
dc.subject Charcoal en
dc.subject Rats en
dc.title Sentinel lymph node biopsy in rats. Comparison between paraffin and frozen section analysis en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation UNIFESP
dc.description.affiliation UNIFESP Pathology Department
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP) Department of Surgery
dc.description.affiliationUnifesp UNIFESP, Pathology Department
dc.description.affiliationUnifesp UNIFESP, Department of Surgery
dc.identifier.file S0102-86502014001300012.pdf
dc.identifier.scielo S0102-86502014001300012
dc.identifier.doi 10.1590/S0102-86502014001300011
dc.description.source SciELO
dc.identifier.wos WOS:000342065000012


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