A manual method to obtain platelet rich plasma

A manual method to obtain platelet rich plasma

Author Marques, Fabiana Paulino Google Scholar
Ingham, Sheila Jean McNeill Autor UNIFESP Google Scholar
Forgas, Andrea Google Scholar
Franciozi, Carlos Eduardo da Silveira Autor UNIFESP Google Scholar
Sasaki, Pedro Henrique Google Scholar
Abdalla, Rene Jorge Autor UNIFESP Google Scholar
Institution Hospital do Coração Knee Institute
Universidade Federal de São Paulo (UNIFESP)
Abstract OBJECTIVE:This study is to report a manual method to obtain platelet rich plasma (PRP).METHODS:For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and platelet rich plasma. Blood and platelet rich plasma samples were sent to the Hospital's Laboratory and platelets and leukocytes were measured.RESULTS:A sample of 637 blood donors was evaluated. The platelet yield efficiency was 86.77% and the increase in platelet concentration factor was 2.89 times. The increase in leukocyte concentration factor was 1.97 times.CONCLUSION:The method described here produces leukocyte-rich and platelet-rich plasma with a high platelet and leukocyte increased factor.Level of Evidence IV, Controlled Laboratory Study.
Keywords Platelet-rich plasma
Blood platelets
Language English
Date 2014-01-01
Published in Acta Ortopédica Brasileira. Sociedade Brasileira de Ortopedia e Traumatologia, v. 22, n. 2, p. 75-77, 2014.
ISSN 1413-7852 (Sherpa/Romeo)
Publisher Sociedade Brasileira de Ortopedia e Traumatologia
Extent 75-77
Origin http://dx.doi.org/10.1590/1413-78522014220200798
Access rights Open access Open Access
Type Article
SciELO ID S1413-78522014000200075 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/8166

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