Nitric oxide levels in cochlear fluids of guinea pig

Nitric oxide levels in cochlear fluids of guinea pig

Author Yonamine, Fernando Kaoru Autor UNIFESP Google Scholar
Onishi, Ektor Tsuneo Autor UNIFESP Google Scholar
Mouro, Margaret Gori Autor UNIFESP Google Scholar
Higa, Elisa Mieko Suemitsu Autor UNIFESP Google Scholar
Cruz, Oswaldo Laércio Mendonça Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract PURPOSE: To describe a technique to obtain guinea pigs cochlear fluids and measure nitric oxide (NO) concentration. METHODS: Six guinea pigs were used and sacrificed. The cochlear fluids collected for measurement of NO, performed by chemiluminescence (NOA 280). RESULTS: Through the chemiluminescence was possible to analyze the concentration of NO in cochlear fluids obtained. Average levels of nitric oxide from guinea pigs was 12.55 µM. CONCLUSION: It is possible to obtain nitric oxide cochlear fluids, with this technique and nitric oxide concentration measure by chemiluminescence, a quantitative and more precise method.
Keywords Nitric Oxide
Cochlea
Guinea Pigs
Language English
Date 2013-05-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 5, p. 397-402, 2013.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 397-402
Origin http://dx.doi.org/10.1590/S0102-86502013000500013
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000319994200013
SciELO ID S0102-86502013000500013 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/7742

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