Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury: immunohistochemical study in rat model

Sildenafil citrate protects skeletal muscle of ischemia-reperfusion injury: immunohistochemical study in rat model

Author Armstrong, Dinani Matoso Fialho de Oliveira Google Scholar
Armstrong, Anderson da Costa Google Scholar
Figueiredo, Regina Célia Bressan Queiroz Google Scholar
Florentino, Joao Eduardo Google Scholar
Saad, Paulo Fernandes Autor UNIFESP Google Scholar
Fox-Talbot, Karen Google Scholar
Halushka, Marc Kenneth Google Scholar
Berkowitz, Dan E. Google Scholar
Taha, Murched Omar Autor UNIFESP Google Scholar
Fagundes, Djalma José Autor UNIFESP Google Scholar
Institution Sao Francisco Valley Federal University Department of Surgery
UNIVASF Department of Cardiology
Research Center Aggeu Magalhaes Department of Microbiology
Pernambuco Federal University
UNIVASF Department of Surgery
Johns Hopkins University Department of Pathology
Johns Hopkins University Department of Anesthesiology and Critical Care Medicine
Universidade Federal de São Paulo (UNIFESP)
Abstract PURPOSE: To investigate the effect of sildenafil citrate (SC) on skeletal muscle ischemia-reperfusion (IR) injury in rats. METHODS: Adult male Wistar rats were randomized into three groups: vehicle-treated control (CTG), sildenafil citrate-treated (SCG), and sham group (SG). CTG and SCG had femoral artery occluded for 6 hours. Saline or 1 mg/kg of SC was given 5.5 hours after occlusion. SG had a similar procedure without artery occlusion. Soleus muscle samples were acquired 4 or 24h after the reperfusion. Immunohistochemistry caspase-3 analysis was used to estimate apoptosis using the apoptotic ratio (computed as positive/negative cells). Wilcoxon rank-sum or Kruskal-Wallis tests were used to assess differences among groups. RESULTS: Eighteen animals were included in the 4h reperfusion groups and 21 animals in the 24h reperfusion groups. The mean apoptotic ratio was 0.18±0.1 for the total cohort; 0.14±0.06 for the 4h reperfusion groups and 0.19±0.08 for the 24h groups (p<0.05). The SCG had lower caspase-3 ratio compared to the control groups at the 24h reperfusion time point (p<0.05). CONCLUSION: Sildenafil citrate administration after the onset of the ischemic injury reduces IR-induced cellular damage in skeletal muscle in this rat hindlimb ischemia model.
Keywords Reperfusion Injury
Muscle, Skeletal
Phosphodiesterase
Inhibitors
Caspase 3
Rats
Language English
Date 2013-04-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 4, p. 282-287, 2013.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 282-287
Origin http://dx.doi.org/10.1590/S0102-86502013000400008
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000317522300008
SciELO ID S0102-86502013000400008 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/7683

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