The influence of sleep deprivation and obesity on DNA damage in female Zucker rats

The influence of sleep deprivation and obesity on DNA damage in female Zucker rats

Author Tenório, Neuli Maria Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Alvarenga, Tathiana Aparecida Autor UNIFESP Google Scholar
Fracalossi, Ana Carolina Cuzzuol Autor UNIFESP Google Scholar
Carlin, Viviane Autor UNIFESP Google Scholar
Hirotsu, Camila Autor UNIFESP Google Scholar
Tufik, Sergio Autor UNIFESP Google Scholar
Andersen, Monica Levy Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract OBJECTIVE: The aim of this study was to evaluate overall genetic damage induced by total sleep deprivation in obese, female Zucker rats of differing ages. METHOD: Lean and obese Zucker rats at 3, 6, and 15 months old were randomly distributed into two groups for each age group: home-cage control and sleep-deprived (N = 5/group). The sleep-deprived groups were deprived sleep by gentle handling for 6 hours, whereas the home-cage control group was allowed to remain undisturbed in their home-cage. At the end of the sleep deprivation period, or after an equivalent amount of time for the home-cage control groups, the rats were brought to an adjacent room and decapitated. The blood, brain, and liver tissue were collected and stored individually to evaluate DNA damage. RESULTS: Significant genetic damage was observed only in 15-month-old rats. Genetic damage was present in the liver cells from sleep-deprived obese rats compared with lean rats in the same condition. Sleep deprivation was associated with genetic damage in brain cells regardless of obesity status. DNA damage was observed in the peripheral blood cells regardless of sleep condition or obesity status. CONCLUSION: Taken together, these results suggest that obesity was associated with genetic damage in liver cells, whereas sleep deprivation was associated with DNA damage in brain cells. These results also indicate that there is no synergistic effect of these noxious conditions on the overall level of genetic damage. In addition, the level of DNA damage was significantly higher in 15-month-old rats compared to younger rats.
Keywords Sleep Deprivation
Obesity
Zucker Rats
DNA Damage
Comet Assay
Gentle Handling
Female
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Associacao Fundo de Incentivo a Pesquisa (AFIP)
Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (CEPID)
Grant number 98/14303-3 e 11/12325-6 e 10/50129-1 e 07/01228-4 e 09/03360-2
Date 2013-01-01
Published in Clinics. Faculdade de Medicina / USP, v. 68, n. 3, p. 385-389, 2013.
ISSN 1807-5932 (Sherpa/Romeo, impact factor)
Publisher Faculdade de Medicina / USP
Extent 385-389
Origin http://dx.doi.org/10.6061/clinics/2013(03)OA16
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000320000800017
SciELO ID S1807-59322013000300017 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/7590

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