Antioxidant supplementation decreases the cell death rate in the prostatic stromal tissue of long-term castrated rats

Antioxidant supplementation decreases the cell death rate in the prostatic stromal tissue of long-term castrated rats

Author Fartes, Guilherme Autor UNIFESP Google Scholar
Lorenzetti, Fábio Autor UNIFESP Google Scholar
Salvador, Larissa Beloti Autor UNIFESP Google Scholar
Ortiz, Valdemar Autor UNIFESP Google Scholar
Dambros, Miriam Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract OBJECTIVE: The purpose of this study was to compare the effects of castration on cell death rate of the adult rat prostates and to evaluate the benefic action of alpha tocopherol supplementation to avoid apoptosis post-orchiectomy. MATERIAL AND METHODS: Thirty male Wistar rats weighing 250-300g were divided into three groups: group I - they were subjected to bilateral orchiectomy and sacrificed eight weeks after the procedure; group II - subjected to bilateral orchiectomy and alpha-tocopherol supplementation for four weeks preceding the procedure; and group III - subjected to bilateral orchiectomy and alpha-tocopherol supplementation for four weeks preceding the procedure and for eight weeks afterwards. At the end of the experiment, the prostatectomy was performed in all rats. The presence of oxidative stress was determined by assaying the blood level of 8-isoprostane and the occurrence of apoptosis was evaluated by identification of active caspase-3 through immunohistochemical analysis. RESULTS: The statistic analysis of active caspase-3 showed that in the long-term castrated group the detection was higher than in groups were the alpha-tocopherol was supplemented (p=0.007). Analysis of 8-isoprostane levels showed higher concentrations of reactive oxygen species in group I compared to other groups (p<0.05). Groups II and III presented active caspase-3 lower than in group I (p<0.05). CONCLUSION: Our exploratory analyses demonstrate a method to study the aging process and its influence on oxidative stress of prostatic tissue and cells death rate. Based on our results we can suggest that alpha tocopherol supplementation can decrease the apoptotic process as well as the oxidative stress levels induced by androgen deprivation of the prostate gland.
Keywords Prostate
Testosterone
Oxidative Stress
alpha-Tocopherol
Apoptosis
Language English
Date 2012-06-01
Published in International braz j urol. Sociedade Brasileira de Urologia, v. 38, n. 3, p. 419-425, 2012.
ISSN 1677-5538 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira de Urologia
Extent 419-425
Origin http://dx.doi.org/10.1590/S1677-55382012000300016
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000314415400027
SciELO ID S1677-55382012000300016 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/7128

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