A liver metalloendopeptidase which degrades the circulating hypotensive peptide hormones bradykinin and atrial natriuretic peptide

A liver metalloendopeptidase which degrades the circulating hypotensive peptide hormones bradykinin and atrial natriuretic peptide

Author Carvalho, K.m. Google Scholar
Nava, R.a. Google Scholar
França, M.s.f. Google Scholar
Medeiros, M.a.s. Google Scholar
Camarão, Gisela Costa Autor UNIFESP Google Scholar
Juliano, Luiz Autor UNIFESP Google Scholar
Institution Universidade Federal do Ceará
Universidade Estadual do Ceará
Universidade Federal de São Paulo (UNIFESP)
Abstract A new metalloendopeptidase was purified to apparent homogeneity from a homogenate of normal human liver using successive steps of chromatography on DEAE-cellulose, hydroxyapatite and Sephacryl S-200. The purified enzyme hydrolyzed the Pro7-Phe8 bond of bradykinin and the Ser25-Tyr26 bond of atrial natriuretic peptide. No cleavage was produced in other peptide hormones such as vasopressin, oxytocin or Met- and Leu-enkephalin. This enzyme activity was inhibited by 1 mM divalent cation chelators such as EDTA, EGTA and o-phenanthroline and was insensitive to 1 µM phosphoramidon and captopril, specific inhibitors of neutral endopeptidase (EC 3.4.24.11) and angiotensin-converting enzyme (EC 3.4.15.1), respectively. With Mr 85 kDa, the enzyme exhibits optimal activity at pH 7.5. The high affinity of this endopeptidase for bradykinin (Km = 10 µM) and for atrial natriuretic peptide (Km = 5 µM) suggests that it may play a physiological role in the inactivation of these circulating hypotensive peptide hormones.
Keywords liver metalloendopeptidase
bradykinin
atrial natriuretic peptide
Language English
Date 1999-01-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 32, n. 1, p. 51-54, 1999.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Associação Brasileira de Divulgação Científica
Extent 51-54
Origin http://dx.doi.org/10.1590/S0100-879X1999000100007
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000078324100007
SciELO ID S0100-879X1999000100007 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/712

Show full item record




File

Name: S0100-879X1999000100007.pdf
Size: 176.8Kb
Format: PDF
Description:
Open file

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account