Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg

Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg

Author Mendes, Rita Gabriela Pedrosa Ribeiro Google Scholar
Gusmão, Michélia Antônia do Nascimento Autor UNIFESP Google Scholar
Maia, Ana Carolina Ribeiro Gomes Google Scholar
Detoni, Michelle de Lima Google Scholar
Porcino, Gabriane Nascimento Google Scholar
Soares, Thais Vieira Google Scholar
Juliano, Maria Aparecida Autor UNIFESP Google Scholar
Juliano, Luiz Autor UNIFESP Google Scholar
Coelho, Paulo Marcos Zech Google Scholar
Lenzi, Henrique Leonel Google Scholar
Faria-Pinto, Priscila Google Scholar
Vasconcelos, Eveline Gomes Google Scholar
Institution Universidade Federal de Juiz de Fora Instituto de Ciências Biológicas Departamento de Bioquímica
Universidade Federal de São Paulo (UNIFESP)
Instituto de Pesquisas René Rachou-Fiocruz
Instituto Oswaldo Cruz-Fiocruz Laboratório de Patologia
Abstract A peptide (SmB2LJ; r175-194) that belongs to a conserved domain from Schistosoma mansoni SmATPDase 2 and is shared with potato apyrase, as predicted by in silico analysis as antigenic, was synthesised and its immunostimulatory property was analysed. When inoculated in BALB/c mice, this peptide induced high levels of SmB2LJ-specific IgG1 and IgG2a subtypes, as detected by enzyme linked immunosorbent assay. In addition, dot blots were found to be positive for immune sera against potato apyrase and SmB2LJ. These results suggest that the conserved domain r175-194 from the S. mansoni SmATPDase 2 is antigenic. Western blots were performed and the anti-SmB2LJ antibody recognised in adult worm (soluble worm antigen preparation) or soluble egg antigen antigenic preparations two bands of approximately 63 and 55 kDa, molecular masses similar to those predicted for adult worm SmATPDase 2. This finding strongly suggests the expression of this same isoform in S. mansoni eggs. To assess localisation of SmATPDase 2, confocal fluorescence microscopy was performed using cryostat sections of infected mouse liver and polyclonal antiserum against SmB2LJ. Positive reactions were identified on the external surface from the miracidium in von Lichtenberg's envelope and, in the outer side of the egg-shell, showing that this soluble isoform is secreted from the S. mansoni eggs.
Keywords Schistosoma mansoni
NTPDase
potato apyrase
peptide
Language English
Date 2011-11-01
Published in Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 106, n. 7, p. 808-813, 2011.
ISSN 0074-0276 (Sherpa/Romeo, impact factor)
Publisher Instituto Oswaldo Cruz, Ministério da Saúde
Extent 808-813
Origin http://dx.doi.org/10.1590/S0074-02762011000700005
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000297789800005
SciELO ID S0074-02762011000700005 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/6685

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