Direct fluorescent antibody assay and polymerase chain reaction for the detection of Chlamydia trachomatis in patients with vernal keratoconjunctivitis

Direct fluorescent antibody assay and polymerase chain reaction for the detection of Chlamydia trachomatis in patients with vernal keratoconjunctivitis

Author Nishiwaki-Dantas, Maria Cristina Google Scholar
Abreu, Mariza Toledo de Autor UNIFESP Google Scholar
Melo, Cynthia Mendonça de Google Scholar
Romero, Ivana Lopes Google Scholar
Belfort, Rubens Neto Autor UNIFESP Google Scholar
Dantas, Paulo Elias Correa Google Scholar
Institution Santa Casa of São Paulo Corneal and External Disease Service Department of Ophthalmology
Universidade Federal de São Paulo (UNIFESP)
Sorocaba Eye Hospital
Abstract OBJECTIVES: To identify Chlamydia trachomatis via polymerase chain reaction and a direct fluorescent antibodyassay in patients with vernal keratoconjunctivitis while comparing the efficacies of both tests for detectingChlamydia trachomatis in these conditions. METHODS: Conjunctival scraping samples were obtained from 177 patients who were divided into two groups: avernal keratoconjunctivitis group (group A) and a control group (group B). The polymerase chain reaction and adirect fluorescent antibody assay were performed. Sensitivity, specificity, receiver operating characteristic curves,and areas under the curve were calculated for both tests in groups A and B. Receiver operating characteristic curveswere plotted using a categorical variable with only two possible outcomes (positive and negative). RESULTS: Statistical analysis revealed a significant association between vernal keratoconjunctivitis and Chlamydia trachomatis infection detected by a direct fluorescent antibody assay with high sensitivity and specificity. Allpatients in group A with positive polymerase chain reactions also presented with positive direct fluorescentantibody assays. CONCLUSION: The association between vernal keratoconjunctivitis and Chlamydia trachomatis infection wasconfirmed by positive direct fluorescent antibody assays in 49.4% of vernal keratoconjunctivitis patients and bypositive polymerase chain reactions in 20% of these patients. The direct fluorescent antibody assay detectedChlamydia trachomatis in a higher number of patients than did the polymerase chain reaction. Although thediagnosis of trachoma is essentially clinical, the disease may not be detected in vernal keratoconjunctivitis patients.Due to the high frequency of chlamydial infection detected in patients with vernal keratoconjunctivitis, we suggestconsidering routine laboratory tests to detect Chlamydia trachomatis in patients with severe and refractory allergicdisease.
Keywords Vernal keratoconjunctivitis
Chlamydia trachomatis
Polymerase chain reaction
Direct fluorescentantibody assay
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2011-01-01
Published in Clinics. Faculdade de Medicina / USP, v. 66, n. 12, p. 2013-2018, 2011.
ISSN 1807-5932 (Sherpa/Romeo, impact factor)
Publisher Faculdade de Medicina / USP
Extent 2013-2018
Origin http://dx.doi.org/10.1590/S1807-59322011001200003
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000298331400003
SciELO ID S1807-59322011001200003 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/6208

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