Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth

Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth

Author Della Torre, Adriana Google Scholar
Albuquerque, Lia de Barros Leite Google Scholar
Farrapo, Nicole Moreira Google Scholar
Oshima-Franco, Yoko Google Scholar
Santos, Marcio Galdino dos Google Scholar
Tavares, Renata Vasques da Silva Google Scholar
Rodas, Andrea Cecilia Dorión Google Scholar
Dal Belo, Cháriston André Google Scholar
Cardoso, Cássia Regina Primila Google Scholar
Varanda, Eliana Aparecida Google Scholar
Groppo, Francisco Carlos Google Scholar
Lopes, Patricia Santos Autor UNIFESP Google Scholar
Institution University of Sorocaba
Federal University of Tocantins
Nuclear and Energy Research Institute
Federal University of Pampa
Universidade Estadual Paulista (UNESP)
Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins). The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test) and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9) and without (-S9) metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P. reticulata extract was mutagenic to TA98 (-S9) and showed signs of mutagenic activity in TA97a and TA102 (both -S9) strains. Micronucleus test CBPI values showed that the endogenous metabolic system increased the number of viable cells when compared to the non-activated samples and the micronucleus frequency increased when the cells were treated in the absence of S9. We concluded that P. reticulata extract may present direct mutagenic properties.
Keywords Ames test
micronucleus test
mutagenicity
plant extract
Plathymenia reticulata
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2011-01-01
Published in Journal of Venomous Animals and Toxins including Tropical Diseases. Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 17, n. 2, p. 190-198, 2011.
ISSN 1678-9199 (Sherpa/Romeo, impact factor)
Publisher Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
Extent 190-198
Origin http://dx.doi.org/10.1590/S1678-91992011000200010
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000290866500010
SciELO ID S1678-91992011000200010 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/6156

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