IL-2, IL-5, TNF-α and IFN-γ mRNA expression in epidermal keratinocytes of systemic lupus erythematosus skin lesions

IL-2, IL-5, TNF-α and IFN-γ mRNA expression in epidermal keratinocytes of systemic lupus erythematosus skin lesions

Author Carneiro, José Ronaldo Matos Autor UNIFESP Google Scholar
Fuzii, Hellen T Google Scholar
Kayser, Cristiane Autor UNIFESP Google Scholar
Alberto, Fernando L Google Scholar
Soares, Fernando Augusto Google Scholar
Sato, Emilia Inoue Autor UNIFESP Google Scholar
Andrade, Luiz Eduardo Coelho Autor UNIFESP Google Scholar
Institution Universidade Federal do Pará
Universidade Federal de São Paulo (UNIFESP)
Fleury Medicine and Health Research and Development Institute
Câncer Hospital
Abstract OBJECTIVE: To analyze cytokine gene expression in keratinocytes from patients with systemic lupus erythematosus (SLE). INTRODUCTION: Keratinocytes represent 95% of epidermal cells and can secrete several cytokines. METHODS: Keratinocytes were obtained by laser microdissection from 21 patients with SLE (10 discoid and 11 acute lesions) at involved and uninvolved sites. All patients were receiving a low/moderate prednisone dose and 18 were receiving chloroquine diphosphate. IL-2, IL-5, TNF-α and IFN-γ gene expression was evaluated by real-time PCR and expressed as the ratio (R) to a pool of skin samples from 12 healthy volunteers. RESULTS: Heterogeneity in cytokine gene expression was found among patients with SLE. Eighteen of 38 valid SLE samples (47%) presented overexpression (R>1) of at least one cytokine. Lesional skin samples tended to show higher cytokine expression than samples from uninvolved skin (p = 0.06). IL-5 and IFN-γ were the most commonly overexpressed cytokines. Samples with cytokine overexpression corresponded to more extensive and severe lesions. Prednisone dose did not differ between samples without cytokine overexpression (15.71±3.45 mg/day) and those with overexpressed cytokines (12.68±5.41 mg/day) (p = 0.216). Samples from all patients not receiving diphosphate chloroquine had at least one overexpressed cytokine. CONCLUSIONS: The heterogeneous keratinocyte cytokine gene expression reflects the complex immunological and inflammatory background in SLE. Patients with severe/extensive skin lesions showed a higher frequency of cytokine gene overexpression. Increased IFN-γ and IL-5 expression suggests that Th1 and Th2 cells are involved in SLE skin inflammation. The possibility that prednisone and antimalarial drugs may have contributed to low cytokine gene expression in some samples cannot be ruled out.
Keywords Cytokines
Gene expression
Interleukins
Lupus
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Brazilian Society of Rheumatology
Grant number FAPESP: 02/2446-1
Date 2011-01-01
Published in Clinics. Faculdade de Medicina / USP, v. 66, n. 1, p. 77-82, 2011.
ISSN 1807-5932 (Sherpa/Romeo, impact factor)
Publisher Faculdade de Medicina / USP
Extent 77-82
Origin http://dx.doi.org/10.1590/S1807-59322011000100014
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000288712700014
SciELO ID S1807-59322011000100014 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/6138

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