Differential diagnosis in primary and metastatic cutaneous melanoma by FT-Raman spectroscopy

Differential diagnosis in primary and metastatic cutaneous melanoma by FT-Raman spectroscopy

Alternative title Diagnóstico diferencial no melanoma primário e metastático por espectroscopia FT-Raman
Author Oliveira, Andrea Fernandes de Autor UNIFESP Google Scholar
Santos, Ivan Dunshee de Abranches Oliveira Autor UNIFESP Google Scholar
Cartaxo, Sidney Bandeira Autor UNIFESP Google Scholar
Bitar, Renata Andrade Autor UNIFESP Google Scholar
Enokihara, Mílvia Maria Simões e Silva Google Scholar
Martinho, Herculano da Silva Google Scholar
Martin, Airton Abrahão Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Pathology Department
Pathology Department Federal University of ABC Head of Center for Human and Natural Sciences (CCNH)
UNIVAP Institute of Research and Development Head of Biomedical Vibrational Spectroscopy Laboratory
Abstract PURPOSE: To qualify the FT-Raman spectral data of primary and metastatic cutaneous melanoma in order to obtain a differential diagnosis. METHODS: Ten normal human skin samples without any clinical or histopathological alterations, ten cutaneous melanoma fragments, and nine lymph node metastasis samples were used; 105, 140 and 126 spectra were obtained respectively. Each sample was divided into 2 or 3 fragments of approximately 2 mm³ and positioned in the Raman spectrometer sample holder in order to obtain the spectra; a monochrome laser light Nd:YAG at 1064 nm was used to excite the inelastic effect. RESULTS: To differentiate the three histopathological groups according to their characteristics extracted from the spectra, data discriminative analysis was undertaken. Phenylalanine, DNA, and Amide-I spectral variables stood out in the differentiation of the three groups. The percentages of correctly classified groups based on Phenylalanine, DNA, and Amide-I spectral features was 93.1%. CONCLUSION: FT-Raman spectroscopy is capable of differentiating melanoma from its metastasis, as well as from normal skin.

OBJETIVO: Qualificar os dados espectrais FT-Raman do melanoma cutâneo primário e metastático e assim realizar o diagnóstico diferencial. MÉTODOS: Foram utilizadas amostras de 10 fragmentos de pele sem alterações clínicas ou histopatológicas, 10 de melanomas cutâneos e 9 de metástases linfonodais; 105, 140 and 126 espectros foram obtidos respectivamente. Cada amostra foi dividida em 2 ou 3 frações de 2 mm³ e posicionada no porta amostras do espectrômetro Raman para obtenção dos espectros, por meio da excitação do espalhamento inelástico pelo laser de Nd:YAG em 1064 nm incididos na amostra. RESULTADOS: Para diferenciar os três grupos formados de acordo com as características fornecidas pelos espectros, realizamos a análise discriminante dos dados. As variáveis espectrais Fenilalanina, DNA e Amida-I se destacaram na capacidade de diferenciação dos três grupos histológicos. A porcentagem de classificação correta utilizando estes critérios foi de 93,1%; o que mostra a eficiência da análise realizada. CONCLUSÃO: A espectroscopia FT-Raman é capaz de diferenciar o melanoma de sua metástase, assim como da pele normal.
Keywords Melanoma
Spectrum Analysis, Raman
Diagnosis
Biopsy
Melanoma
Análise Espectral Raman
Diagnóstico
Biópsia
Language English
Date 2010-10-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 25, n. 5, p. 434-439, 2010.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 434-439
Origin http://dx.doi.org/10.1590/S0102-86502010000500009
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000282219500009
SciELO ID S0102-86502010000500009 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/5982

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