Effect of the exposure to fine inhalable particulate matter (pm2.5) on sperm functional quality of mice

Effect of the exposure to fine inhalable particulate matter (pm2.5) on sperm functional quality of mice

Author Intasqui, Paula Google Scholar
Tamashiro, Letícia Kaory Google Scholar
Yariwake, Victor Yuji Google Scholar
Souza, Rosana Xavier Google Scholar
Kanashiro, Camila Matie Google Scholar
Veras, Mariana Matera Google Scholar
Abstract Objective: To evaluate the effect of exposure to pollution (fine inhalable particulate matter - PM2.5) from the city of São Paulo on sperm functional quality. Design: Male isogenic BALB/c mice were used, distributed in two groups, control (n=6) and polluted air (n=8). For the polluted air group, after weaning (21 days), animals were daily exposed to 600 μg/m3 of PM2,5 for 96 days in an Ambient Particle Concentrator (APC). Control group was simultaneously exposed to filtered air in the APC. On postnatal day 118, animals were sacrificed (isoflurane overdose), body was weighted and the epididymis were collected. Materials and Methods: Sperm obtained from the cauda epididymis were used for the evaluation of motility, mitochondrial activity (DAB staining), acrosome integrity (PNA staining), DNA fragmentation (alkaline comet assay), oxidative stress (DHE staining) and cell viability (PI staining). Groups were compared using an unpaired Student’s t test (p<0.05). Results: Groups did not differ regarding body weight, and sperm motility. Furthermore, air pollution did not alter sperm functional quality (Table 1). Conclusions: Exposure to high concentrations of PM2.5 does not affect sperm motility and functional parameters.
Keywords Air pollution
DNA Fragmentation
Oxidative Stress
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2020-09-01
ISSN 0015-0282 (Sherpa/Romeo)
Publisher American Society for Reproductive Medicine
Extent E17-E18
Origin https://www.fertstert.org/article/S0015-0282(20)30828-1/fulltext
Access rights Open access Open Access
Type Conference paper
URI https://repositorio.unifesp.br/handle/11600/59038

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