Oxidative origin of sperm DNA fragmentation in the adult varicocele

Oxidative origin of sperm DNA fragmentation in the adult varicocele

Author Jeremias, Jessica Timóteo Google Scholar
Belardin, Larissa Berloffa Google Scholar
Okada, Fatima Kazue Google Scholar
Antoniassi, Mariana Pereira Google Scholar
Fraietta, Renato Google Scholar
Bertolla, Ricardo Pimenta Google Scholar
Intasqui, Paula Google Scholar
Abstract Purpose: Sperm DNA fragmentation is a major cellular mechanism underlying varicocele-related male infertility. However, the type of DNA fragmentation - whether oxidative or of another nature - remains unknown. Thus, the aim of this study was to evaluate single- and double-stranded sperm DNA fragmentation, and oxidative-induced sperm DNA damage in men with varicocele. Materials and Methods: A cross-sectional study was performed, including 94 normozoospermic adults, of which 39 men without varicocele (controls) and 55 men with varicocele grades II or III, uni- or bilaterally. All men collected semen by masturbation. After semen analysis, the remaining volume was used for evaluation of three types of sperm DNA damage: (i) total DNA fragmentation, using an alkaline comet assay, (ii) double-stranded DNA fragmentation, using a neutral comet assay, and (iii) oxidative DNA damage, using an alkaline comet assay associated with the DNA glycosylase formamidopyrimidine enzyme. In each assay, percentage of sperm with any degree of DNA fragmentation, and with high DNA fragmentation were compared between the groups using an unpaired Student’s t test or a Mann-Whitney test. Results: The varicocele group presented a higher rate of sperm with fragmented DNA (both any and high DNA fragmentation), considering single-stranded DNA fragmentation, double-stranded DNA fragmentation, or a combination of both, as well as oxidativeinduced DNA fragmentation. Conclusions: Patients with varicocele have an increase in sperm DNA fragmentation levels, particularly in oxidative stress-induced sperm DNA damage.
Keywords DNA Fragmentation
Infertility, Male
Oxidative Stress
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2020-09-10
ISSN 1677-6119 (Sherpa/Romeo)
1677-5538 (Sherpa/Romeo)
Publisher Brazilian Society of Urology– SBU
Extent Ahead of print
Origin https://www.intbrazjurol.com.br/pdf/vol47n02/IBJU20190827.pdf
Access rights Open access Open Access
Type Article
URI https://repositorio.unifesp.br/handle/11600/59037

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