Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line

Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line

Author Ribeiro, Hercules Ferreira Autor UNIFESP Google Scholar
Alcântara, D.f.a. Google Scholar
Matos, L.a. Google Scholar
Sousa, J.m.c. Google Scholar
Leal, Mariana Ferreira Autor UNIFESP Google Scholar
Smith, Marilia de Arruda Cardoso Autor UNIFESP Google Scholar
Burbano, Rommel Rodríguez Autor UNIFESP Google Scholar
Bahia, Marcelo de Oliveira Google Scholar
Institution Universidade Federal do Pará Instituto de Ciências Biológicas Laboratório de Citogenética Humana
Universidade Federal de São Paulo (UNIFESP)
Abstract Gastric cancer is the fourth most frequent type of cancer and the second cause of cancer mortality worldwide. The genetic alterations described so far for gastric carcinomas include amplifications and mutations of the c-ERBB2, KRAS, MET, TP53, and c-MYC genes. Chromosomal instability described for gastric cancer includes gains and losses of whole chromosomes or parts of them and these events might lead to oncogene overexpression, showing the need for a better understanding of the cytogenetic aspects of this neoplasia. Very few gastric carcinoma cell lines have been isolated. The establishment and characterization of the biological properties of gastric cancer cell lines is a powerful tool to gather information about the evolution of this malignancy, and also to test new therapeutic approaches. The present study characterized cytogenetically PG-100, the first commercially available gastric cancer cell line derived from a Brazilian patient who had a gastric adenocarcinoma, using GTG banding and fluorescent in situ hybridization to determine MYC amplification. Twenty metaphases were karyotyped; 19 (95%) of them presented chromosome 8 trisomy, where the MYC gene is located, and 17 (85%) presented a deletion in the 17p region, where the TP53 is located. These are common findings for gastric carcinomas, validating PG100 as an experimental model for this neoplasia. Eighty-six percent of 200 cells analyzed by fluorescent in situ hybridization presented MYC overexpression. Less frequent findings, such as 5p deletions and trisomy 16, open new perspectives for the study of this tumor.
Keywords Carcinogenesis
Chromosomal abnormalities
Gastric cancer
Oncogenes
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number CNPq: 20/2007
CNPq: 550885/2007-2
Date 2010-08-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 43, n. 8, p. 717-721, 2010.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Associação Brasileira de Divulgação Científica
Extent 717-721
Origin http://dx.doi.org/10.1590/S0100-879X2010007500068
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000283261800004
SciELO ID S0100-879X2010000800004 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/5877

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