Angiomirs expression profiling in diffuse large B-Cell lymphoma

Angiomirs expression profiling in diffuse large B-Cell lymphoma

Author Borges, Natalia M. Autor UNIFESP Google Scholar
Elias, Marcela do Vale Autor UNIFESP Google Scholar
Fook-Alves, Veruska L. Autor UNIFESP Google Scholar
Andrade, Tathiana A. Autor UNIFESP Google Scholar
de Conti, Marina Lourenco Autor UNIFESP Google Scholar
Macedo, Mariana Petaccia Google Scholar
Begnami, Maria Dirlei Google Scholar
Campos, Antonio Hugo J. F. M. Google Scholar
Etto, Leina Yukari Autor UNIFESP Google Scholar
Bortoluzzo, Adriana Bruscato Google Scholar
Alves, Antonio C. Autor UNIFESP Google Scholar
Young, Ken H. Google Scholar
Colleoni, Gisele W. B. Autor UNIFESP Google Scholar
Abstract Despite advances in treatment, 30% of diffuse large B-cell lymphoma (DLBCL) cases are refractory or relapse after chemoimmunotherapy. Currently, the relationship between angiogenesis and angiomiRs in DLBCL is unknown. We classified 84 DLBCL cases according to stromal signatures and evaluated the expression of pro-and antiangiomiRs in paraffin embedded tissues of DLBCL and correlated them with microvascular density (MVD). 40% of cases were classified as stromal-1, 50% as stromal-2 and 10% were not classified. We observed increased expression of proangiomiRs Let-7f, miR-17, miR-18a, miR-19b, miR-126, miR-130a, miR-210, miR-296 and miR-378 in 14%, 57%, 30%, 45%, 12%, 12%, 56%, 58% and 48% of the cases, respectively. Among antiangiomiRs we found decreased expression of miR-16, miR-20b, miR-92a, miR-221 and miR-328 in, respectively, 27%, 71%, 2%, 44% and 11%. We found association between increased expression of proangiomiRs miR-126 and miR-130a and antiangiomiR miR-328 and the subtype non-GCB. We found higher levels of the antiangiomiRs miR-16, miR-221 and miR-328 in patients with low MVD and stromal-1 signature. IPI and CD34 confirmed independent impact on survival of the study group. None of the above angiomiRs showed significance as biomarker in an independent serum samples cohort of patients and controls. In conclusion, we confirmed association between antiangiomiRs miR-16, miR-221 and miR-328 and stromal-1 signature. Four angiomiRs emerged as potential therapeutic targets: proangiomiRs miR-17, miR-210 and miR-296 and antiangiomiR miR-20b. Although the four microRNAs seem to be important in DLBCL pathogenesis, they were not predictive of DLBCL onset or relapse in the serum independent cohort.
Keywords lymphoma
angiogenesis
microRNAs
xmlui.dri2xhtml.METS-1.0.item-coverage Orchard Park
Language English
Sponsor Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil
Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), Brazil
Grant number FAPESP: 2010/17668-6
Date 2016
Published in Oncotarget. Orchard Park, v. 7, n. 4, p. 4806-4816, 2016.
ISSN 1949-2553 (Sherpa/Romeo, impact factor)
Publisher Impact Journals Llc
Extent 4806-4816
Origin https://doi.org/10.18632/oncotarget.6624
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000369952400086
URI https://repositorio.unifesp.br/handle/11600/58673

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