Diazepam effects on aversive memory retrieval and extinction: Role of anxiety levels

Diazepam effects on aversive memory retrieval and extinction: Role of anxiety levels

Author Leao, Anderson H. F. F. Google Scholar
Cabral, Alicia Google Scholar
Izidio, Geison S. Google Scholar
Ribeiro, Alessandra M. Autor UNIFESP Google Scholar
Silva, Regina H. Autor UNIFESP Google Scholar
Abstract Benzodiazepines (BDZs) are anxiolytic drugs that impair memory acquisition. Previous studies using the plus maze discriminative avoidance task (PMDAT, which assesses memory and anxiety concomitantly) indicated that the effects of BDZs on anxiety and acquisition are related to each other. The possible influence of the anxiolytic action of BDZs on their effects on memory retrieval and extinction are poorly understood. This is relevant considering the relationship between aversive memories and anxiety disorders. We designed a modified protocol of PMDAT that evaluates anxiety during retrieval and extinction of the task. Male Wistar rats were trained in the PMDAT (plus-maze with two open and two enclosed arms) using a standard or a modified protocol. In the standard protocol, the aversive stimuli were presented in one of the enclosed arms during training, and the animal had free access to the whole apparatus. In the modified protocol, the open arms were blocked with glass walls. Twenty-four hours after training, the animals subjected to each of the protocols were treated with saline or 2.0 mg/kg of diazepam (DZP) 30 min before the test. There was a third session in the maze (retest) 24 h after the test. During the test, DZP impaired and improved retrieval in rats that had been trained in the standard and the modified protocol when compared to the respective saline-treated groups. In addition, treatment with DZP prior to the test induced anxiolysis, but only in the animals that were not pre-exposed to the open arms of the apparatus (modified protocol). In these animals, DZP impaired extinction, which was evaluated during retest session. The impairing effect of DZP on extinction seems to be related to its anxiolytic action during the test (extinction learning). Further, we suggest that aversive memory retrieval depends on both the treatment and the arousal elicited by exposure to the apparatus. (C) 2015 Elsevier Inc. All rights reserved.
Keywords Memory
One-trial tolerance
xmlui.dri2xhtml.METS-1.0.item-coverage Oxford
Language English
Sponsor Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ)
Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
Fundacao de Apoio a Pesquisa do Estado do Rio Grande do Norte (FAPERN)
Grant number CNPq: 473548/2012-7
Date 2016
Published in Pharmacology Biochemistry And Behavior. Oxford, v. 141, p. 42-49, 2016.
ISSN 0091-3057 (Sherpa/Romeo, impact factor)
Publisher Pergamon-Elsevier Science Ltd
Extent 42-49
Origin https://doi.org/10.1016/j.pbb.2015.11.012
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000369466100005
URI https://repositorio.unifesp.br/handle/11600/58612

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