Pilocarpine-induced epilepsy alters the expression and daily variation of the nuclear receptor ROR alpha in the hippocampus of rats

Pilocarpine-induced epilepsy alters the expression and daily variation of the nuclear receptor ROR alpha in the hippocampus of rats

Author Rocha, Anna Karynna Alves de Alencar Autor UNIFESP Google Scholar
de Lima, Eliangela Autor UNIFESP Google Scholar
do Amaral, Fernanda Gaspar Google Scholar
Peres, Rafael Google Scholar
Cipolla-Neto, Jose Google Scholar
Amado, Debora Autor UNIFESP Google Scholar
Abstract It is widely known that there is an increase in the inflammatory responses and oxidative stress in temporal lobe epilepsy (TLE). Further, the seizures follow a circadian rhythmicity. Retinoic acid receptor-related orphan receptor alpha (ROR alpha) is related to anti-inflammatory and antioxidant enzyme expression and is part of the machinery of the biological clock and circadian rhythms. However, the participation of ROR alpha in this neurological disorder has not been studied. The aim of this study was to evaluate the ROR alpha mRNA and protein content profiles in the hippocampus of rats submitted to a pilocarpine-induced epilepsy model at different time points throughout the 24-h light-dark cycle analyzing the influence of the circadian rhythm in the expression pattern during the acute, silent, and chronic phases of the experimental model. Real-time PCR and immunohistochemistry results showed that ROR alpha mRNA and protein expressions were globally reduced in both acute and silent phases of the pilocarpine model. However, 60 days after the pilocarpine-induced status epilepticus (chronic phase), the mRNA expression was similar to the control except for the time point 3 h after the lights were turned off, and no differences were found in immunohistochemistry. Our results indicate that the status epilepticus induced by pilocarpine is able to change the expression and daily variation of ROR alpha in the rat hippocampal area during the acute and silent phases. These findings enhance our understanding of the circadian pattern present in seizures as well as facilitate strategies for the treatment of seizures. (C) 2015 Elsevier Inc. All rights reserved.
Keywords Epilepsy
Pilocarpine
ROR alpha receptor
xmlui.dri2xhtml.METS-1.0.item-coverage San Diego
Language English
Sponsor CAPES
FAPESP
INNT
CNPq (Brazil)
Date 2016
Published in Epilepsy & Behavior. San Diego, v. 55, p. 38-46, 2016.
ISSN 1525-5050 (Sherpa/Romeo, impact factor)
Publisher Academic Press Inc Elsevier Science
Extent 38-46
Origin https://doi.org/10.1016/j.yebeh.2015.11.026
Access rights Closed access
Type Article
Web of Science ID WOS:000369685700007
URI https://repositorio.unifesp.br/handle/11600/58471

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