FT-Raman spectroscopy for the differentiation between cutaneous melanoma and pigmented nevus

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dc.contributor.author Cartaxo, Sidney Bandeira [UNIFESP]
dc.contributor.author Santos, Ivan Dunshee de Abranches Oliveira [UNIFESP]
dc.contributor.author Bitar, Renata Andrade [UNIFESP]
dc.contributor.author Oliveira, Andréa Fernandes [UNIFESP]
dc.contributor.author Ferreira, Lydia Masako [UNIFESP]
dc.contributor.author Martinho, Herculano Silva
dc.contributor.author Martin, Airton Abrahão
dc.date.accessioned 2015-06-14T13:41:48Z
dc.date.available 2015-06-14T13:41:48Z
dc.date.issued 2010-08-01
dc.identifier http://dx.doi.org/10.1590/S0102-86502010000400010
dc.identifier.citation Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 25, n. 4, p. 351-356, 2010.
dc.identifier.issn 0102-8650
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/5842
dc.description.abstract Cutaneous melanoma is the most aggressive type of skin cancer and Ft-Raman spectroscopy has been studied as a potential method that could be a real alternative for early diagnosis of neoplasms. PURPOSE: To qualify the spectral FT-Raman data, in order to differentiate cutaneous melanoma and pigmented nevus. METHODS: For this study, 10 samples of cutaneous melanoma, 9 samples of pigmented nevi, and 10 samples of normal skin were obtained by incisional biopsies performed during plastic surgeries ex vivo, immediately after removing the surgical sample. RESULTS: The FT-Raman spectra of each group presented a high correlation between the elements of the same group, thus favoring the elaboration of spectral averages. When analyzing the spectral standard of each group, the normal skin standard did not show a significant variation between the spectra; the standard of the pigmented nevi group showed significant variation, and the cutaneous melanoma group also showed variation. Through univariate analysis, specific bands were detected for each vibrational mode identified. The discriminatory analysis of the data showed a 75.3% efficiency of the differentiation between the three groups studied. CONCLUSION: The vibrational modes Polysaccharides, Tyrosine and Amide-I differentiated the melanoma from the pigmented nevus. en
dc.description.abstract O melanoma cutâneo é o câncer de pele mais agressivo, e a espectroscopia FT-Raman tem sido estudada como um método em potencial que pode ser uma verdadeira alternativa no diagnóstico precoce de neoplasias. OBJETIVO: Qualificar os dados espectrais FT-Raman de modo a diferenciar melanoma cutâneo de nevo pigmentado. MÉTODOS: Foram utilizadas 10 amostras de melanoma cutâneo, obtidas por meio de biopsias incisionais realizadas ex-vivo; nove amostras de nevo pigmentado e 10 amostras de pele normal foram coletadas durante cirurgias plásticas. RESULTADOS: Os espectros FT-Raman de cada grupo diagnóstico apresentaram alta correlação entre os elementos do mesmo grupo, o que favoreceu a realização das médias espectrais. Analisando o padrão espectral de cada grupo, o de pele normal não mostrou grande variação entre os espectros; o de nevo pigmentado apresentou variação notável e, o grupo melanoma primário também indicou variação. Por meio de análise univariada foram identificadas bandas específicas para cada modo vibracional identificado. A análise discriminante aos dados mostrou 75,3% de eficiência na diferenciação entre os três grupos estudados. CONCLUSÃO: Os modos vibracionais Polissacarídeos (Banda I), Tirosina (Banda 6) e Amida I (Banda 10) diferenciaram o melanoma do nevo pigmentado. pt
dc.format.extent 351-356
dc.language.iso eng
dc.publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartof Acta Cirurgica Brasileira
dc.rights Acesso aberto
dc.subject Melanoma en
dc.subject Spectrum Analysis, Raman en
dc.subject Nevus, Pigmented en
dc.subject Biopsy en
dc.subject Melanoma pt
dc.subject Análise Espectral Raman pt
dc.subject Nevo Pigmentado pt
dc.subject Biópsia pt
dc.title FT-Raman spectroscopy for the differentiation between cutaneous melanoma and pigmented nevus en
dc.title.alternative Espectroscopia FT-Raman na diferenciação entre melanoma cutâneo e nevo pigmentado pt
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution UFABC Center for Human and Natural Sciences
dc.contributor.institution UNIVAP Institute of Research and Development - IP&D Head of Biomedical Vibrational Spectroscopy Laboratory
dc.description.affiliation UNIFESP Post-Graduate Program in Plastic Surgery
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP) Department of Surgery Head of Plastic Surgery Division
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP) Department of Surgery Division and Chairwoman
dc.description.affiliation UFABC Center for Human and Natural Sciences
dc.description.affiliation UNIVAP Institute of Research and Development - IP&D Head of Biomedical Vibrational Spectroscopy Laboratory
dc.description.affiliationUnifesp UNIFESP, Post-Graduate Program in Plastic Surgery
dc.description.affiliationUnifesp UNIFESP, Department of Surgery Head of Plastic Surgery Division
dc.description.affiliationUnifesp UNIFESP, Department of Surgery Division and Chairwoman
dc.identifier.file S0102-86502010000400010.pdf
dc.identifier.scielo S0102-86502010000400010
dc.identifier.doi 10.1590/S0102-86502010000400010
dc.description.source SciELO
dc.identifier.wos WOS:000280607200009



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