Quantitative evaluation of experimental choroidal neovascularization by confocal scanning laser ophthalmoscopy: fluorescein angiogram parallels heparan sulfate proteoglycan expression

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dc.contributor.author Regatieri, Caio Vinicius Saito [UNIFESP]
dc.contributor.author Dreyfuss, Juliana Luporini [UNIFESP]
dc.contributor.author Melo, G.b. [UNIFESP]
dc.contributor.author Lavinsky, Daniel [UNIFESP]
dc.contributor.author Hossaka, S.k. [UNIFESP]
dc.contributor.author Rodrigues, Eduardo Buchele [UNIFESP]
dc.contributor.author Farah, Michel Eid [UNIFESP]
dc.contributor.author Nader, Helena Bonciani [UNIFESP]
dc.contributor.author Maia, Maurício [UNIFESP]
dc.date.accessioned 2015-06-14T13:41:47Z
dc.date.available 2015-06-14T13:41:47Z
dc.date.issued 2010-07-01
dc.identifier http://dx.doi.org/10.1590/S0100-879X2010007500043
dc.identifier.citation Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 43, n. 7, p. 627-633, 2010.
dc.identifier.issn 0100-879X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/5832
dc.description.abstract The objective of the present study was to develop a quantitative method to evaluate laser-induced choroidal neovascularization (CNV) in a rat model using Heidelberg Retina Angiograph 2 (HRA2) imaging. The expression of two heparan sulfate proteoglycans (HSPG) related to inflammation and angiogenesis was also investigated. CNV lesions were induced with argon laser in 21 heterozygous Zucker rats and after three weeks a fluorescein angiogram and autofluorescence exams were performed using HRA2. The area and greatest linear dimension were measured by two observers not aware of the protocol. Bland-Altman plots showed agreement between the observers, suggesting that the technique was reproducible. After fluorescein angiogram, HSPG (perlecan and syndecan-4) were analyzed by real-time RT-PCR and immunohistochemistry. There was a significant increase in the expression of perlecan and syndecan-4 (P < 0.0001) in retinas bearing CNV lesions compared to control retinas. The expression of these two HSPG increased with increasing CNV area. Immunohistochemistry demonstrated that the rat retina damaged with laser shots presented increased expression of perlecan and syndecan-4. Moreover, we observed that the overexpression occurred in the outer layer of the retina, which is related to choroidal damage. It was possible to develop a standardized quantitative method to evaluate CNV in a rat model using HRA2. In addition, we presented data indicating that the expression of HSPG parallels the area of CNV lesion. The understanding of these events offers opportunities for studies of new therapeutic interventions targeting these HSPG. en
dc.format.extent 627-633
dc.language.iso eng
dc.publisher Associação Brasileira de Divulgação Científica
dc.relation.ispartof Brazilian Journal of Medical and Biological Research
dc.rights Acesso aberto
dc.subject Choroidal neovascularization en
dc.subject Fluorescein angiogram en
dc.subject Proteoglycans en
dc.subject Angiogenesis en
dc.subject Confocal scanning laser ophthalmoscope en
dc.title Quantitative evaluation of experimental choroidal neovascularization by confocal scanning laser ophthalmoscopy: fluorescein angiogram parallels heparan sulfate proteoglycan expression en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP) Departamento de Oftalmologia
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP) Departamento de Bioquímica
dc.description.affiliationUnifesp UNIFESP, Depto. de Oftalmologia
dc.description.affiliationUnifesp UNIFESP, Depto. de Bioquímica
dc.identifier.file S0100-879X2010000700004.pdf
dc.identifier.scielo S0100-879X2010000700004
dc.identifier.doi 10.1590/S0100-879X2010007500043
dc.description.source SciELO



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