Quantitative evaluation of experimental choroidal neovascularization by confocal scanning laser ophthalmoscopy: fluorescein angiogram parallels heparan sulfate proteoglycan expression

Quantitative evaluation of experimental choroidal neovascularization by confocal scanning laser ophthalmoscopy: fluorescein angiogram parallels heparan sulfate proteoglycan expression

Author Regatieri, Caio Vinicius Saito Autor UNIFESP Google Scholar
Dreyfuss, Juliana Luporini Autor UNIFESP Google Scholar
Melo, G.b. Autor UNIFESP Google Scholar
Lavinsky, Daniel Autor UNIFESP Google Scholar
Hossaka, S.k. Autor UNIFESP Google Scholar
Rodrigues, Eduardo Buchele Autor UNIFESP Google Scholar
Farah, Michel Eid Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Maia, Maurício Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The objective of the present study was to develop a quantitative method to evaluate laser-induced choroidal neovascularization (CNV) in a rat model using Heidelberg Retina Angiograph 2 (HRA2) imaging. The expression of two heparan sulfate proteoglycans (HSPG) related to inflammation and angiogenesis was also investigated. CNV lesions were induced with argon laser in 21 heterozygous Zucker rats and after three weeks a fluorescein angiogram and autofluorescence exams were performed using HRA2. The area and greatest linear dimension were measured by two observers not aware of the protocol. Bland-Altman plots showed agreement between the observers, suggesting that the technique was reproducible. After fluorescein angiogram, HSPG (perlecan and syndecan-4) were analyzed by real-time RT-PCR and immunohistochemistry. There was a significant increase in the expression of perlecan and syndecan-4 (P < 0.0001) in retinas bearing CNV lesions compared to control retinas. The expression of these two HSPG increased with increasing CNV area. Immunohistochemistry demonstrated that the rat retina damaged with laser shots presented increased expression of perlecan and syndecan-4. Moreover, we observed that the overexpression occurred in the outer layer of the retina, which is related to choroidal damage. It was possible to develop a standardized quantitative method to evaluate CNV in a rat model using HRA2. In addition, we presented data indicating that the expression of HSPG parallels the area of CNV lesion. The understanding of these events offers opportunities for studies of new therapeutic interventions targeting these HSPG.
Keywords Choroidal neovascularization
Fluorescein angiogram
Proteoglycans
Angiogenesis
Confocal scanning laser ophthalmoscope
Language English
Date 2010-07-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 43, n. 7, p. 627-633, 2010.
ISSN 0100-879X (Sherpa/Romeo)
Publisher Associação Brasileira de Divulgação Científica
Extent 627-633
Origin http://dx.doi.org/10.1590/S0100-879X2010007500043
Access rights Open access Open Access
Type Article
SciELO ID S0100-879X2010000700004 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/5832

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