Photobiomodulation therapy action in wound repair skin induced in aged rats old: time course of biomarkers inflammatory and repair

Show simple item record

dc.contributor.author Fiorio, Franciane Barbieri
dc.contributor.author dos Santos, Solange Almeida
dc.contributor.author de Melo Rambo, Caroline Sobral
dc.contributor.author Dalbosco, Camila Guerra
dc.contributor.author Serra, Andrey Jorge
dc.contributor.author de Melo, Brunno Lemes [UNIFESP]
dc.contributor.author Pinto Leal-Junior, Ernesto Cesar
dc.contributor.author Camillo de Carvalho, Paulo de Tarso
dc.date.accessioned 2020-09-01T13:21:30Z
dc.date.available 2020-09-01T13:21:30Z
dc.date.issued 2017
dc.identifier http://dx.doi.org/10.1007/s10103-017-2254-2
dc.identifier.citation Lasers In Medical Science. London, v. 32, n. 8, p. 1769-1782, 2017.
dc.identifier.issn 0268-8921
dc.identifier.uri https://repositorio.unifesp.br/handle/11600/58307
dc.description.abstract Previous studies have discussed an inverse correlation between age and wound healing, because it relates to the association of aging with a gradual decrease in healing capacity. Treatment with photobiomodulation therapy (PBMT) improves wound healing by inducing increases in mitotic activity, numbers of fibroblasts, collagen synthesis, and neovascularization. Therefore, this study aimed to evaluate the effects of PBMT in cutaneous wound healing in aged rats. A punch biopsy of 8 mm in diameter was performed to produce a skin wound. The study included 45 male rats, of which 15 were young (30 days) and 30 were elderly (500 days). The 45 animals were distributed into 3 experimental groups, which were subjected to skin wounds and 1 aged group received PBMT, with a 30-mW laser beam (power density of 1.07 W/cm(2)), beam area of 0.028 cm(2), and lambda 660 nm produced through active phosphide Gallium-Aluminum-Indio (InGaAIP). The PBMT application took the form of a single-point transcutaneous method, with a total energy of 2 joules per wound site, energy density of 72 J/cm(2), and time of 1 min and 7 s. Analysis was performed to verify the effect of PBMT on the quantity of collagen I and III, metalloproteinase 3 and 9 (MMP-3 and MMP-9), tissue inhibitor of metalloproteinase-2 (TIMP-2) and of vascular endothelial growth factor (VEGF) at the wound site by immunohistochemistry, cytokine-induced neutrophil chemoattractant (CINC)-1, by enzyme-linked immunosorbent assay (ELISA) and interleukin (IL)-6 real-time polymerase chain reaction (RT-PCR). That we conclude LLLT is effective in the modulation of inflammatory mediators IL-6, CINC-1, VEGF, MMP-3, MMP-9 and TIMP-2 as well as increased collagen production in aged animals during different phases of the tissue regeneration process. However, the effects of PBMT obtained in the aged animals (aged LLLT group) suggest that new dosimetries should be tested to achieve better results. en
dc.description.sponsorship Sao Paulo Research Foundation (FAPESP)
dc.description.sponsorship National Council for Scientific and Technological Development - CNPq
dc.format.extent 1769-1782
dc.language.iso eng
dc.publisher Springer London Ltd
dc.relation.ispartof Lasers In Medical Science
dc.rights Acesso aberto
dc.subject Wound healing en
dc.subject Photobiomodulation therapy en
dc.subject Inflammatory mediators en
dc.subject Age en
dc.title Photobiomodulation therapy action in wound repair skin induced in aged rats old: time course of biomarkers inflammatory and repair en
dc.type Artigo
dc.description.affiliation Univ Nove de Julho UNINOVE, Postgrad Program Rehabil Sci, Sao Paulo, SP, Brazil
dc.description.affiliation Chapeco Univ UNOCHAPECO, Hlth Sci Ctr, Chapeco, SC, Brazil
dc.description.affiliation Nove de Julho Univ UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, Rua Vergueiro 235, Sao Paulo, SP, Brazil
dc.description.affiliation Univ Fed Sao Paulo UNIFESP, Postgrad Program Med Cardiol, Sao Paulo, SP, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo UNIFESP, Postgrad Program Med Cardiol, Sao Paulo, SP, Brazil
dc.description.sponsorshipID FAPESP: 2015/13677-4
dc.description.sponsorshipID CNPq: 309065/2015-1
dc.identifier.doi 10.1007/s10103-017-2254-2
dc.description.source Web of Science
dc.identifier.wos WOS:000413620900008
dc.coverage London
dc.citation.volume 32
dc.citation.issue 8



File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account