Photobiomodulation therapy action in wound repair skin induced in aged rats old: time course of biomarkers inflammatory and repair

Photobiomodulation therapy action in wound repair skin induced in aged rats old: time course of biomarkers inflammatory and repair

Author Fiorio, Franciane Barbieri Google Scholar
dos Santos, Solange Almeida Google Scholar
de Melo Rambo, Caroline Sobral Google Scholar
Dalbosco, Camila Guerra Google Scholar
Serra, Andrey Jorge Google Scholar
de Melo, Brunno Lemes Autor UNIFESP Google Scholar
Pinto Leal-Junior, Ernesto Cesar Google Scholar
Camillo de Carvalho, Paulo de Tarso Google Scholar
Abstract Previous studies have discussed an inverse correlation between age and wound healing, because it relates to the association of aging with a gradual decrease in healing capacity. Treatment with photobiomodulation therapy (PBMT) improves wound healing by inducing increases in mitotic activity, numbers of fibroblasts, collagen synthesis, and neovascularization. Therefore, this study aimed to evaluate the effects of PBMT in cutaneous wound healing in aged rats. A punch biopsy of 8 mm in diameter was performed to produce a skin wound. The study included 45 male rats, of which 15 were young (30 days) and 30 were elderly (500 days). The 45 animals were distributed into 3 experimental groups, which were subjected to skin wounds and 1 aged group received PBMT, with a 30-mW laser beam (power density of 1.07 W/cm(2)), beam area of 0.028 cm(2), and lambda 660 nm produced through active phosphide Gallium-Aluminum-Indio (InGaAIP). The PBMT application took the form of a single-point transcutaneous method, with a total energy of 2 joules per wound site, energy density of 72 J/cm(2), and time of 1 min and 7 s. Analysis was performed to verify the effect of PBMT on the quantity of collagen I and III, metalloproteinase 3 and 9 (MMP-3 and MMP-9), tissue inhibitor of metalloproteinase-2 (TIMP-2) and of vascular endothelial growth factor (VEGF) at the wound site by immunohistochemistry, cytokine-induced neutrophil chemoattractant (CINC)-1, by enzyme-linked immunosorbent assay (ELISA) and interleukin (IL)-6 real-time polymerase chain reaction (RT-PCR). That we conclude LLLT is effective in the modulation of inflammatory mediators IL-6, CINC-1, VEGF, MMP-3, MMP-9 and TIMP-2 as well as increased collagen production in aged animals during different phases of the tissue regeneration process. However, the effects of PBMT obtained in the aged animals (aged LLLT group) suggest that new dosimetries should be tested to achieve better results.
Keywords Wound healing
Photobiomodulation therapy
Inflammatory mediators
Age
xmlui.dri2xhtml.METS-1.0.item-coverage London
Language English
Sponsor Sao Paulo Research Foundation (FAPESP)
National Council for Scientific and Technological Development - CNPq
Grant number FAPESP: 2015/13677-4
CNPq: 309065/2015-1
Date 2017
Published in Lasers In Medical Science. London, v. 32, n. 8, p. 1769-1782, 2017.
ISSN 0268-8921 (Sherpa/Romeo, impact factor)
Publisher Springer London Ltd
Extent 1769-1782
Origin http://dx.doi.org/10.1007/s10103-017-2254-2
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000413620900008
URI https://repositorio.unifesp.br/handle/11600/58307

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