In vivo confocal Raman spectroscopy for intrinsic aging and photoaging assessment

In vivo confocal Raman spectroscopy for intrinsic aging and photoaging assessment

Author Nasser Caetano, Livia de Vasconcelos Autor UNIFESP Google Scholar
Mendes, Thiago de Oliveira Google Scholar
Bagatin, Edileia Autor UNIFESP Google Scholar
Miot, Helio Amante Google Scholar
Marques Soares, Juliana Laudiceia Autor UNIFESP Google Scholar
Enokihara, Milvia Maria Simoes e Silva Autor UNIFESP Google Scholar
Martin, Airton Abrahao Google Scholar
Abstract Background: In vivo confocal Raman spectroscopy is a non-invasive method to assess either the epidermis or the dermis composition. Few studies have focused on dermis collagen alterations through intrinsic aging and photoaging. Objective: This study evaluated the in vivo Raman spectra from the dermis of a photoexposed site versus a non-photoexposed region in different age groups, and evaluated the correlation between peak intensities and age, photoaging score and the amount of collagen assessed with histology and high frequency ultrasound (HFUS). Methods: Fifteen volunteers aged 28-82 years were divided into three groups according to forearm photoaging degree. In vivo Raman spectra from the dermis were collected on the dorsal forearm (chronically photoexposed skin) and on the proximal medial arm (non-photoexposed skin). Crosssectional images of the skin were obtained using a 20 MHz ultrasound unit exactly on the same sites, which were further submitted to punch biopsies for histologic study (collagen I immunohistochemistry, picrosirius red staining and Verhoeff). Principal Component Analysis (PCA) and Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) were taken in the spectral region of 796 cm(-1) -996 cm(-1) to determine its potential to discriminate between different groups. The Spearman rank correlation coefficient of individual peak intensities and ratios with age, clinical score and the amount of collagen assessed by ultrasound and histology were calculated. Results: PCA of pairs of groups and OPLS-DA could discriminate the intrinsically from the photoaged skin and the young group from the elderly one, with important contribution of the 938 cm(-1) and 855 cm(-1) peaks intensities. The intensity of the peaks in 855 cm(-1) and/or 938 cm(-1) presented moderate correlation with age (rho = 0.579, p = 0.049) and moderate to high inverse correlation with HFUS echogenicity (rho = -0.710, p = 0.010) and collagen I immunohistochemistry (rho = -0.833, p = 0.005) in the non-photoexposed region. The I-1275/I-1450 intensities ratio presented moderate to high correlation coefficients with age (rho = -0.730, p = 0.007), photoaging score (rho = -0.594, p = 0.042), HFUS echogenicity (rho = 0.760, p < 0.001) and histology (collagen I immunohistochemistry (rho = 0.643, p = 0.024), picrosirius (rho = 0.773, p = 0.005) and Verhoeff (rho = -0.727, p = 0.011)) in the photoexposed site. Conclusion: The wavenumber region between 798 and 994 cm(-1) is useful for the analysis of dermal collagen alterations through the intrinsic aging process, while photoaging is better assessed by the I-1275/I-1450 intensities ratio. This is the first skin aging study to show a correlation between Raman peaks and the amount of collagen assessed by HFUS- and histology. (C) 2017 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.
Keywords High frequency ultrasound
Histology
Photoaging
Raman spectroscopy
Skin aging
xmlui.dri2xhtml.METS-1.0.item-coverage Clare
Language English
Sponsor FUNADERSP, an organisation of the Sao Paulo regional office of the Brazilian Society of Dermatology
Grant number FUNADERSP: 001/2013
Date 2017
Published in Journal Of Dermatological Science. Clare, v. 88, n. 2, p. 199-206, 2017.
ISSN 0923-1811 (Sherpa/Romeo, impact factor)
Publisher Elsevier Ireland Ltd
Extent 199-206
Origin http://dx.doi.org/10.1016/j.jdermsci.2017.07.011
Access rights Closed access
Type Article
Web of Science ID WOS:000416193500006
URI https://repositorio.unifesp.br/handle/11600/58260

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