Effect of atenolol pre-treatment in heart damage in a model of intestinal ischemia-reperfusion

Effect of atenolol pre-treatment in heart damage in a model of intestinal ischemia-reperfusion

Author Okada, Mieko Autor UNIFESP Google Scholar
Reis Falcao, Luiz Fernando Autor UNIFESP Google Scholar
Ferez, David Autor UNIFESP Google Scholar
Martins, Jose Luiz Autor UNIFESP Google Scholar
Errante, Paolo Ruggero Autor UNIFESP Google Scholar
Menezes Rodrigues, Francisco Sandro Autor UNIFESP Google Scholar
Caricati-Neto, Afonso Autor UNIFESP Google Scholar
Marinho, Marcia Google Scholar
Fenelon, Guilherme Autor UNIFESP Google Scholar
Oliveira-Junior, Itamar Souza Autor UNIFESP Google Scholar
Abstract Purpose: To investigate the effects of atenolol in inflammatory mediator and oxidative stress in a myocardial injury by intestinal ischemia/reperfusion in rat model. Methods: Adult Wistar male rats were randomly (n= 8), anesthetized and divided in: Sham: submitted to operation only; group SS+ IR: intravenous saline infusion following superior mesenteric artery occlusion during 60 minutes (ischemia) and open for 120 minutes (reperfusion); group AT+ IR: intravenous atenolol infusion (2 mg/kg) following superior mesenteric artery occlusion during 60 minutes (ischemia) and open for 120 minutes (reperfusion); and group AT+ I+ AT+ R: intravenous atenolol infusion following superior mesenteric artery occlusion during 60 minutes (ischemia) and in the time 45 minutes other atenolol doses were administrated and the artery was open for 120 minutes (reperfusion), all animals were submitted to muscular relaxation for mechanical ventilation. In the end of experiment the animals were euthanized and the hearts tissue were morphology analyzed by histology and malondialdehyde by ELISA, and the plasma were analyzed for tumor necrosis factor-alpha by ELISA. Results: The group SS+ IR demonstrated the higher malondialdehyde levels when compared with the atenolol treated-groups (p= 0.001) in the heart tissue. The tumor necrosis factoralpha level in plasma decrease in the treated groups when compared with SS+ IR group (p= 0.001). Histology analyses demonstrate pyknosis, edema, cellular vacuolization, presence of inflammatory infiltrate and band contraction in the heart tissue of the rats. Conclusion: Atenolol significantly reduce the degree of cardiac damage after intestinal ischemia-reperfusion.
Keywords Adrenergic Antagonists
Atenolol
Ischemia
Reperfusion
Cytokines
Oxidative Stress
Rats
xmlui.dri2xhtml.METS-1.0.item-coverage Sao Paulo
Language English
Sponsor FAPESP
Date 2017
Published in Acta Cirurgica Brasileira. Sao Paulo, v. 32, n. 11, p. 964-972, 2017.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Acta Cirurgica Brasileira
Extent 964-972
Origin http://dx.doi.org/10.1590/s0102-865020170110000008
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000417846700008
SciELO ID S0102-86502017001100964 (statistics in SciELO)
URI https://repositorio.unifesp.br/handle/11600/58222

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