The expression of endothelial and inducible nitric oxide synthase and apoptosis in intestinal ischemia and reperfusion injury under the action of ischemic preconditioning and pentoxifylline

The expression of endothelial and inducible nitric oxide synthase and apoptosis in intestinal ischemia and reperfusion injury under the action of ischemic preconditioning and pentoxifylline

Author Ribeiro de Oliveira, Teresinha Regina Google Scholar
de Oliveira, Geraldo Ferreira Google Scholar
Simoes, Ricardo Santos Google Scholar
Feitosa, Suellen Maurim Autor UNIFESP Google Scholar
Tikazawa, Eduardo Hiroshi Google Scholar
Monteiro, Hugo Pequeno Autor UNIFESP Google Scholar
Fagundes, Djalma Jose Autor UNIFESP Google Scholar
Taha, Murched Omar Autor UNIFESP Google Scholar
Abstract Purpose: To investigate the expression of nitric oxide synthase (NOS) and apoptosis associated with ischemic preconditioning (IPC) and pentoxifylline (PTX) in intestinal ischemia (I) and reperfusion (R) injury. Methods: Thirty male rats were assigned to 5 groups: (CG), no clamping of the superior mesenteric artery (90 minutes); (IR-SS) saline + ischemia (30 minutes) + reperfusion (60 minutes); (IR-PTX) PTX + ischemia (30 minutes) + reperfusion (60 minutes); (IPC-IR-SS) 5 minutes of ischemia + 5 minutes of reperfusion (IPC) + saline + I(30 minutes)+ R(60 minutes); and (IPC-IR-PTX) IPC + PTX + I(30 minutes)+ R(60 minutes). Results: The application of IPC and PTX showed a significantly lower immunohistochemistry reaction for active caspase-3 (P< 0.05) compared to IR+ SS. The number of cells immunoreactive to BCL-2 was higher in the IR-PTX group (P> 0.05). The NOS-2 expression (qRTPCR) in the IRPTX group (P< 0.05) was higher than the values for the IPC+ IR-SS and IPC-IR-PTX groups. The NOS-3 expression was significantly upper in the IPC-IR-PTX group than in the CG (P< 0.05), the IR-SS (P< 0.05) and the IR-PTX (P< 0.05) groups. Conclusions: The BCL-2 and active caspase-3 showed beneficial effects on PTX and IPC. The expression of NOS-2 and NOS-3 in the IPC and IPC-PTX groups showed no synergistic effect.
Keywords Ischemic Preconditioning
Pentoxifylline
Ischemia
Reperfusion Injury
Nitric Oxide Synthase
Apoptosis
Rats
xmlui.dri2xhtml.METS-1.0.item-coverage Sao Paulo
Language English
Sponsor Ministerio da Educacao do Brasil, UFGD
Date 2017
Published in Acta Cirurgica Brasileira. Sao Paulo, v. 32, n. 11, p. 935-948, 2017.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Acta Cirurgica Brasileira
Extent 935-948
Origin http://dx.doi.org/10.1590/s0102-865020170110000005
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000417846700005
SciELO ID S0102-86502017001100935 (statistics in SciELO)
URI https://repositorio.unifesp.br/handle/11600/58221

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