Hyperbaric oxygenation and the genic expression related to oxidative stress in the heart of mice during intestinal ischemia and reperfusion

Hyperbaric oxygenation and the genic expression related to oxidative stress in the heart of mice during intestinal ischemia and reperfusion

Author Somaio Neto, Frederico Google Scholar
Ikejir, Adauto Tsutomo Google Scholar
Bertoletto, Paulo Roberto Google Scholar
Chaves, Jose Carlos Google Scholar
Teruya, Roberto Google Scholar
Fagundes, Djalma Jose Autor UNIFESP Google Scholar
Abstract Purpose: To investigate the effects of hyperbaric oxygenation (HBO) on intestinal ischemia and reperfusion (IR) injury, we evaluated the expression of 84 genes related to oxidative stress and the antioxidant response in mouse hearts. Methods: Four groups were subjected to 60 minutes of intestinal ischemia followed by 60 minutes of reperfusion: IRG, ischemia and reperfusion group without HBO; HBO-IG, which received HBO during ischemia; HBO-RG, which received HBO during reperfusion; and HBOIRG, which received HBO during ischemia and reperfusion. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes. The (RT-qPCR) method was applied. Genes with expression levels three times below or above the threshold cycle were considered significantly hypoexpressed or hyperexpressed, respectively (Student's t-test p< 0.05). Results: Eight genes (9.52%) were hyperexpressed in the IRG. When the HBO groups were compared to the IRG, we found a decrease in the expression of eight genes in the HBO-IG, five genes in the HBO-RG, and seven genes in the HBO-IRG. Conclusion: The reduction in the expression of genes related to oxidative stress and antioxidant defense following HBO in mouse hearts resulting from intestinal IR injury was more favorable during the ischemic period than during the reperfusion period.
Keywords Gene Expression
Reperfusion Injury
Hyperbaric Oxygenation
Oxidative Stress
Mice
xmlui.dri2xhtml.METS-1.0.item-coverage Sao Paulo
Language English
Date 2017
Published in Acta Cirurgica Brasileira. Sao Paulo, v. 32, n. 11, p. 913-923, 2017.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Acta Cirurgica Brasileira
Extent 913-923
Origin http://dx.doi.org/10.1590/s0102-865020170110000003
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000417846700003
SciELO ID S0102-86502017001100913 (statistics in SciELO)
URI https://repositorio.unifesp.br/handle/11600/58220

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