Altered mitochondrial function, calcium signaling, and catecholamine release in chromaffin cells of diabetic and SHR rats

Altered mitochondrial function, calcium signaling, and catecholamine release in chromaffin cells of diabetic and SHR rats

Author Musial, Diego C. Autor UNIFESP Google Scholar
Bomfim, Guilherme H. Autor UNIFESP Google Scholar
Arranz-Tagarro, Juan A. Google Scholar
Mendez-Lopez, Iago Google Scholar
Miranda-Ferreira, Regiane Autor UNIFESP Google Scholar
Jurkiewicz, Aron Autor UNIFESP Google Scholar
Jurkiewicz, Neide H. Autor UNIFESP Google Scholar
Garcia, Antonio G. Google Scholar
Padin, Juan F. Google Scholar
Abstract Comorbidity of diabetes and hypertension is frequent. Here, we have performed a comparative study in three animal models namely, normotensive Wistar Kyoto (WKY) rats, streptozotocin-induced diabetic rats (STZ), and spontaneously hypertensive rats (SHR). With respect WKY rats, we have found the following alterations in adrenal chromaffin cells from STZ and SHR rats: (1) diminished Ca2+ currents; (2) augmented [Ca2+](c) elevations and catecholamine release in cells stimulated with angiotensin II or high K+; (3) unchanged expression of angiotensin II receptors AT(1) and AT(2); (4) higher density of secretory vesicles at subplasmalemmal sites; (5) mitochondria with lower cristae density that were partially depolarized; and (6) lower whole cell ATP content. These alterations may have their origin in (i) an augmented capacity of the endoplasmic reticulum Ca2+ store likely due to (ii) impaired mitochondrial Ca2+ uptake; (iii) augmented high-[Ca2+](c) microdomains at subplasmalemmal sites secondary to augmented calcium-induce calcium release and to inositol tris-phosphate receptor mediated enhanced Ca2+ mobilization from the endoplasmic reticulum; and (iv) augmented vesicle pool. These alterations seem to be common to the two models of human hypertension here explored, STZ diabetic rats and SHR hypertensive rats.
Keywords Diabetes
Hypertension
Spontaneously hypertensive rat
Chromaffin cells
Angiotensin
Mitochondria
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor Ministerio de Economia y Competitividad, Spain
CABICYC
UAM/Bioiberica, Spain
Fundacion Teofilo Hernando, Madrid, Spain
Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
Grant number Ministerio de Economia y Competitividad, Spain: SAF 2013-44108
Ministerio de Economia y Competitividad, Spain: FPI BES-2014-069005
CAPES: BEX 8477/13-2
Date 2017
Published in European Journal Of Pharmacology. Amsterdam, v. 815, p. 416-426, 2017.
ISSN 0014-2999 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 416-426
Origin http://dx.doi.org/10.1016/j.ejphar.2017.09.045
Access rights Closed access
Type Article
Web of Science ID WOS:000413780700048
URI https://repositorio.unifesp.br/handle/11600/58171

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