Effect of Concentrated Apple Extract on Experimental Colitis Induced by Acetic Acid

Effect of Concentrated Apple Extract on Experimental Colitis Induced by Acetic Acid

Author Pastrelo, Maurcio Mercaldi Autor UNIFESP Google Scholar
Dias Ribeiro, Carla Caroline Autor UNIFESP Google Scholar
Duarte, Joselmo Willamys Autor UNIFESP Google Scholar
Bioago Gollucke, Andrea Pitelli Autor UNIFESP Google Scholar
Artigiani-Neto, Ricardo Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Miszputen, Sender Jankiel Autor UNIFESP Google Scholar
Fujiyama Oshima, Celina Tizuko Autor UNIFESP Google Scholar
Ribeiro Paiotti, Ana Paula Autor UNIFESP Google Scholar
Abstract Reactive oxygen and nitrogen species (ROS/RNS) play a crucial role in inflammatory bowel disease (IBD) exacerbating the chronic inflammatory process. Endogenous and diet antioxidants can neutralize these compounds. The apple is widely consumed, with several antioxidant activity compounds. The present study evaluated the effects of concentrated apple extract (CAE) in acetic acid induced colitis. 29 Wistar male rats were randomized into 5 groups. G1-Sham/saline solution, G2-CAE/control, G3-acetic acid/control, G4-curative-CAE treatment and G5-preventive-CAE treatment. Eight days later, the animals were euthanized and the colonic segment resected for macroscopic and histological analysis. Gene expression was evaluated for inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), catalase and copper and zinc superoxide dismutase (CuZnSOD) by quantitative real time PCR, while protein expression was assessed for iNOS, COX-2 and 8-hydroxy-20-deoxyguanosine (8-OHdG) via immunohistochemistry. The groups G3, G4 and G5 had weight loss, while G5 had weight increase at the end of the experiment. The treatment with CAE reduced the macroscopic and microscopic injury, decreased iNOS mRNA expression and increased CuZnSOD mRNA expression in animals with induced acetic acid-colitis. The findings of the present study suggest that CAE treatment exerts an antioxidant role by downregulating iNOS and upregulating CuZnSOD.
Keywords Experimental colitis
apple extract
antioxidant
iNOS
CuZnSOD
xmlui.dri2xhtml.METS-1.0.item-coverage Babol
Language English
Sponsor CAPES
FAPESP
Grant number FAPESP: 2013/26845-7
Date 2017
Published in International Journal Of Molecular And Cellular Medicine. Babol, v. 6, n. 1, p. 38-49, 2017.
ISSN 2251-9637 (Sherpa/Romeo, impact factor)
Publisher Cellular & Molecular Biology Research Center
Extent 38-49
Origin http://dx.doi.org/10.22088/acadpub.BUMS.6.1.38
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000403138600005
URI https://repositorio.unifesp.br/handle/11600/58157

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