Neurochemical Changes and c-Fos Mapping in the Brain after Carisbamate Treatment of Rats Subjected to Lithium-Pilocarpine-Induced Status Epilepticus

Neurochemical Changes and c-Fos Mapping in the Brain after Carisbamate Treatment of Rats Subjected to Lithium-Pilocarpine-Induced Status Epilepticus

Author Marques-Carneiro, Jose Eduardo Autor UNIFESP Google Scholar
Nehlig, Astrid Google Scholar
Cassel, Jean-Christophe Google Scholar
Ferreira Castro-Neto, Eduardo Autor UNIFESP Google Scholar
Litzahn, Julia Julie Autor UNIFESP Google Scholar
de Vasconcelos, Anne Pereira Google Scholar
Naffah-Mazacoratti, Maria da Graca Autor UNIFESP Google Scholar
da Silva Fernandes, Maria Jose Autor UNIFESP Google Scholar
Abstract The administration of lithium-pilocarpine (LiPilo) in adult rats is a validated model reproducing the main clinical and neuropathological features of temporal lobe epilepsy (TLE). Previous studies have shown that carisbamate (CRS) has the property of modifying epileptogenesis in this model. When treated with CRS, about 50% of rats undergoing LiPilo status epilepticus (SE) develop non-convulsive seizures (NCS) instead of convulsive ones (commonly observed in TLE). The goal of this work was to determine some of the early changes that occur after CRS administration, as they could be involved in the insult- and epileptogenesis-modifying effects of CRS. Thus, we performed high-performance liquid chromatography (HPLC) to quantify levels of amino acids and monoamines, and c-Fos immunohistochemical labeling to map cerebral activation during seizures. Comparing rats treated one hour after SE onset with saline (CT), CRS, or diazepam (DZP), HPLC showed that 4 h after SE onset, dopamine (DA), norepinephrine (NE), and GABA levels were normal, whereas serotonin levels were increased. Using c-Fos labeling, we demonstrated increased activity in thalamic mediodorsal (MD) and laterodorsal (LD) nuclei in rats treated with CRS. In summary, at early times, CRS seems to modulate excitability by acting on some monoamine levels and increasing activity of MD and LD thalamic nuclei, suggesting a possible involvement of these nuclei in insult- and/or epileptogenesis-modifying effects of CRS.
Keywords carisbamate 1
temporal-lobe epilepsy 2
brain activity 3
xmlui.dri2xhtml.METS-1.0.item-coverage Basel
Language English
Sponsor Coordenacao de Aperfeicoamento de Pessoal deNivel Superior-CAPES-Brasil
Fundacao de Amparo a Pesquisa no Estado de Sao Paulo-FAPESP
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq
Fundacao de Apoio a Unifesp-FAP-UNIFESP, Brazil
Date 2017
Published in Pharmaceuticals. Basel, v. 10, n. 4, p. -, 2017.
ISSN 1424-8247 (Sherpa/Romeo, impact factor)
Publisher Mdpi Ag
Extent -
Origin http://dx.doi.org/10.3390/ph10040085
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000419241100009
URI https://repositorio.unifesp.br/handle/11600/58064

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