Facial nerve identification with fluorescent dye in rats

Facial nerve identification with fluorescent dye in rats

Author de Melo, Giulianno Molina Autor UNIFESP Google Scholar
Cervantes, Onivaldo Autor UNIFESP Google Scholar
Covolan, Luciene Autor UNIFESP Google Scholar
Baptista, Heloisa Allegro Autor UNIFESP Google Scholar
Ferreira, Elenn Soares Autor UNIFESP Google Scholar
Abrahao, Marcio Autor UNIFESP Google Scholar
Abstract PURPOSE: The parotidectomy technique still has an elevated paresis and paralysis index, lowering patient life's quality. The correct identification of the facial nerve can prevent nerve damage. Fluorescent dye identifies nerves in experimental studies but only few articles focused its use on facial nerve study in parotidectomies. We aimed to stain the rat facial nerve with fluorescent dye to facilitate visualization and dissection in order to prevent injuries. METHODS: Forty adult male Wistar rats were submitted to facial injection of saline solution (Gsf-control group, 10) or fluorescent dye solution (Gdye group, 30) followed by parotidectomy preserving the facial nerve, measuring the time for localization and facility of localization (LocTime and LFN). Nerve function was assessed using the Vibrissae Movements (PMV) and Eyelid Closure Motion (PFP) scores. RESULTS: Nerve localization was faster in Gdye group, with 83% Easy LFN rate. The Gdye group presented with low nerve injury degree and better PMV and PFP scores, with high sensitivity and accuracy. CONCLUSIONS: This experimental method of facial nerve fluorescence was effective for intraoperative nerve visualization, identification and preservation. The technique may be used in future facial nerve studies, translated to humans, contributing to the optimization of parotid surgery in the near future.
Keywords Facial Nerve
Fluorescent Dyes
Parotid Gland
Rats
xmlui.dri2xhtml.METS-1.0.item-coverage Sao Paulo
Language English
Sponsor CAPES
Date 2016
Published in Acta Cirurgica Brasileira. Sao Paulo, v. 31, n. 2, p. 92-102, 2016.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Acta Cirurgica Brasileira
Extent 92-102
Origin http://dx.doi.org/10.1590/S0102-865020160020000003
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000372808700003
SciELO ID S0102-86502016000200092 (statistics in SciELO)
URI https://repositorio.unifesp.br/handle/11600/58002

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