Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy

Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy

Author Lopim, Glauber Menezes Autor UNIFESP Google Scholar
Campos, Diego Vannucci Autor UNIFESP Google Scholar
da Silva, Sergio Gomes Google Scholar
de Almeida, Alexandre Aparecido Autor UNIFESP Google Scholar
Lent, Roberto Google Scholar
Cavalheiro, Esper Abrao Autor UNIFESP Google Scholar
Arida, Ricardo Mario Autor UNIFESP Google Scholar
Abstract The relationship between seizure frequency and cell death has been a subject of controversy. To tackle this issue, we determined the frequency of seizures and the total number of hippocampal cells throughout the life of rats with epilepsy using the pilocarpine model. Seizure frequency varied in animals with epilepsy according to which period of life they were in, with a progressive increase in the number of seizures until 180 days (sixth months) of epileptic life followed by a decrease (330 days-eleventh month) and subsequently stabilization of seizures. Cell counts by means of isotropic fractionation showed a reduction in the number of hippocampal neuronal cells following 30, 90, 180 and 360 days of spontaneous recurrent seizures (SRS) in rats compared to their controls (about 25%-30% of neuronal cell reduction). In addition, animals with 360 days of SRS showed a reduction in the number of neuronal cells when compared with animals with 90 and 180 days of seizures. The total number of hippocampal non-neuronal cells was reduced in rats with epilepsy after 30 days of SRS, but no significant alteration was observed on the 90th, 180th and 360th days. The total number of neuronal cells was negatively correlated with seizure frequency, indicating an association between occurrence of epileptic seizures throughout life and neuronal loss. In sum, our results add novel data to the literature concerning the time-course of SRS and hippocampal cell number throughout epileptic life. (C) 2016 Elsevier B.V. All rights reserved.
Keywords Epilepsy
Seizure frequency
Hippocampus
Neuronal cell
Non-neuronal cell
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor CAPES
FAPESP
CNPq
Instituto Nacional de Neurociencia Translacional (INNT)
Date 2016
Published in Brain Research. Amsterdam, v. 1634, p. 179-186, 2016.
ISSN 0006-8993 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 179-186
Origin http://dx.doi.org/10.1016/j.brainres.2015.12.055
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000372688300018
URI https://repositorio.unifesp.br/handle/11600/57873

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