Association between the seminal plasma proteome and sperm functional traits

Association between the seminal plasma proteome and sperm functional traits

Author Intasqui, Paula Autor UNIFESP Google Scholar
Camargo, Mariana Autor UNIFESP Google Scholar
Antoniassi, Mariana Pereira Autor UNIFESP Google Scholar
Cedenho, Agnaldo Pereira Autor UNIFESP Google Scholar
Carvalho, Valdemir Melechco Google Scholar
Morais Cardozo, Karina Helena Google Scholar
Zylbersztejn, Daniel Suslik Autor UNIFESP Google Scholar
Bertolla, Ricardo Pimenta Autor UNIFESP Google Scholar
Abstract Objective: To analyze the seminal plasma proteome and biological functions associated with sperm functional alterations. Design: Cross-sectional study. Setting: University andrology and research laboratories. Patient(s): A total of 156 normozoospermic men. Intervention(s): Sperm mitochondrial activity, acrosome integrity, and DNA fragmentation were evaluated in a semen aliquot. Remaining semen was centrifuged, and seminal plasma was utilized for proteomic analysis (liquid chromatography-tandem mass spectrometry). Patients were divided into percentiles (15%) to form the following groups: substudy 1, high (control, n = 26) and low (study, n = 23) sperm mitochondrial activity; substudy 2, high (control, n = 23) and low (study, n = 22) sperm acrosome integrity; and substudy 3, low (control, n = 22) and high (study, n = 22) sperm DNA fragmentation. Groups were compared using univariate and multivariate analyses. Differentially expressed proteins were used for functional enrichment analysis. Main Outcome Measure(s): Seminal plasma proteome and postgenomic pathways are associated with several sperm functional traits. Result(s): In total, 506, 493, and 464 proteins were observed in substudies 1, 2, and 3, respectively. Enriched functions in substudy 1 were intramolecular oxidoreductase activity, aminoglycans catabolism, endopeptidases inhibition, lysosomes, and acute-phase response (study group). In substudy 2, main enriched functions were phospholipase inhibition, arachidonic acid metabolism, exocytosis, regulation of acute inflammation, response to hydrogen peroxide, and lysosomal transport (study group). In substudy 3, enriched functions were prostaglandin biosynthesis and fatty acid binding (study group). We proposed eight, six, and eight seminal biomarkers for substudies 1, 2, and 3, respectively. Conclusion(s): Seminal plasma proteome reflects sperm mitochondrial activity reduction, acrosome damage, and DNA fragmentation, with several postgenomic functions related to these alterations. (C) 2016 by American Society for Reproductive Medicine.
Keywords Acrosome
DNA damage
mitochondria
proteomics
semen
xmlui.dri2xhtml.METS-1.0.item-coverage New York
Language English
Sponsor Fleury projects
National Council for Scientific and Technological Development (CNPq)
Sao Paulo Research Foundation (FAPESP)
Grant number CNPq: 472941/2012-7
FAPESP: 2012/1463107
Date 2016
Published in Fertility And Sterility. New York, v. 105, n. 3, p. 617-628, 2016.
ISSN 0015-0282 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Inc
Extent 617-628
Origin http://dx.doi.org/10.1016/j.fertnstert.2015.11.005
Access rights Closed access
Type Article
Web of Science ID WOS:000373406300014
URI https://repositorio.unifesp.br/handle/11600/57855

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