L-Arginine Modulates Intestinal Inflammation in Rats Submitted to Mesenteric Ischemia-Reperfusion Injury

L-Arginine Modulates Intestinal Inflammation in Rats Submitted to Mesenteric Ischemia-Reperfusion Injury

Author Taha, M. O. Autor UNIFESP Google Scholar
de Oliveira, J. V. Google Scholar
Dias Borges, M. Autor UNIFESP Google Scholar
de Lucca Melo, F. Autor UNIFESP Google Scholar
Gualtieri, F. G. Autor UNIFESP Google Scholar
e Silva Aidar, A. L. Autor UNIFESP Google Scholar
Pacheco, R. L. Autor UNIFESP Google Scholar
de Melo Alexandre e Silva, T. Google Scholar
Klajner, R. K. Google Scholar
Iuamoto, L. R. Google Scholar
Munhoz Torres, L. Google Scholar
Morais Mendes de Paula, B. J. Google Scholar
de Campos, K. Google Scholar
Souza de Oliveira, I., Jr. Autor UNIFESP Google Scholar
Fagundes, D. J. Autor UNIFESP Google Scholar
Abstract Background. The goal of this study was to investigate whether exogenous offer of L-arginine (LARG) modulates the gene expression of intestinal dysfunction caused by ischemia and reperfusion. Methods. Eighteen Wistar-EPM1 male rats (250-300 g) were anesthetized and subjected to laparotomy. The superior mesenteric vessels were exposed, and the rats were randomized into 3 groups (n = 6): the control group (CG), with no superior mesenteric artery interruption; the ischemia/reperfusion group (IRG), with 60 minutes of ischemia and 120 minutes of reperfusion and saline injections; and the L-arginine group (IRG + LARG), with L-arginine injected in the femoral vein 5 minutes before ischemia, 5 minutes after reperfusion, and after 55 minutes of reperfusion. The total RNA was extracted and purified from samples of the small intestine. The concentration of each total RNA sample was determined by using spectrophotometry. The first-strand complementary DNA (cDNA) was synthesized in equal amounts of cDNA and the Master Mix SYBR Green qPCR Mastermix (SABiosciences, a Qiagen Company, Frederick, Md). Amounts of cDNA and Master Mix SYBR Green qPCR Mastermix were distributed to each well of the polymerase chain reaction microarray plate containing the predispensed gene-specific primer sets for Bax and Bc12. Each sample was evaluated in triplicate, and the Student t test was applied to validate the homogeneity of each gene expression reaction (P < .05). Results. The gene expression of Bax in IRG (+1.48) was significantly higher than in IRG-LARG (+9.69); the expression of Bc12L1 in IRG (+1.01) was significantly higher than IRG-LARG (+22.89). Conclusions. The apoptotic cell pathway of 2 protagonists showed that LARG improves the gene expression of anti-apoptotic Bc1211 (Bc12-like 1) more than the pro-apoptotic Bax (Bc12-associated X protein).
xmlui.dri2xhtml.METS-1.0.item-coverage New York
Language English
Date 2016
Published in Transplantation Proceedings. New York, v. 48, n. 2, p. 512-515, 2016.
ISSN 0041-1345 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Inc
Extent 512-515
Origin http://dx.doi.org/10.1016/j.transproceed.2015.12.063
Access rights Closed access
Type Article
Web of Science ID WOS:000375240300052
URI https://repositorio.unifesp.br/handle/11600/57840

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