CdSe magic-sized quantum dots incorporated in biomembrane models at the air-water interface composed of components of tumorigenic and non-tumorigenic cells

CdSe magic-sized quantum dots incorporated in biomembrane models at the air-water interface composed of components of tumorigenic and non-tumorigenic cells

Author Goto, Thiago E. Autor UNIFESP Google Scholar
Lopes, Carla C. Autor UNIFESP Google Scholar
Nader, Helena B. Autor UNIFESP Google Scholar
Silva, Anielle C. A. Google Scholar
Dantas, Noeli O. Google Scholar
Siqueira, Jose R., Jr. Google Scholar
Caseli, Luciano Autor UNIFESP Google Scholar
Abstract Cadmium selenide (CdSe) magic-sized quantum dots (MSQDs) are semiconductor nanocrystals with stable luminescence that are feasible for biomedical applications, especially for in vivo and in vitro imaging of tumor cells. In this work, we investigated the specific interaction of CdSe MSQDs with tumorigenic and non-tumorigenic cells using Langmuir monolayers and Langmuir-Blodgett (LB) films of lipids as membrane models for diagnosis of cancerous cells. Surface pressure-area isotherms and polarization modulation reflection-absorption spectroscopy (PM-IRRAS) showed an intrinsic interaction between the quantum dots, inserted in the aqueous subphase, and Langmuir monolayers constituted either of selected lipids or of tumorigenic and non-tumorigenic cell extracts. The films were transferred to solid supports to obtain microscopic images, providing information on their morphology. Similarity between films with different compositions representing cell membranes, with or without the quantum dots, was evaluated by atomic force microscopy (AFM) and confocal microscopy. This study demonstrates that the affinity of quantum dots for models representing cancer cells permits the use of these systems as devices for cancer diagnosis. (C) 2016 Elsevier B.V. All rights reserved.
Keywords CdSe magic-sized quantum dots
Tumorigenic and non-tumorigenic cells
Langmuir-Blodgett ultrathin films
Cancer diagnosis
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor CNPq
FAPEMIG
FAPESP
Grant number CNPq: 477668/2013-5
CNPq: 470890/2012-6
FAPEMIG: APQ-01763-13
FAPESP: 2015/23446-0
Date 2016
Published in Biochimica Et Biophysica Acta-Biomembranes. Amsterdam, v. 1858, n. 7, p. 1533-1540, 2016.
ISSN 0005-2736 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 1533-1540
Origin http://dx.doi.org/10.1016/j.bbamem.2016.04.009
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000377233000013
URI https://repositorio.unifesp.br/handle/11600/57719

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account