Effect of High- and Low-Frequency Transcutaneous Electrical Nerve Stimulation on Angiogenesis and Myofibroblast Proliferation in Acute Excisional Wounds in Rat Skin

Effect of High- and Low-Frequency Transcutaneous Electrical Nerve Stimulation on Angiogenesis and Myofibroblast Proliferation in Acute Excisional Wounds in Rat Skin

Author Machado, Aline F. P. Autor UNIFESP Google Scholar
Liebano, Richard E. Autor UNIFESP Google Scholar
Furtado, Fabianne Autor UNIFESP Google Scholar
Hochman, Bernardo Autor UNIFESP Google Scholar
Ferreira, Lydia M. Autor UNIFESP Google Scholar
Abstract OBJECTIVE: This study evaluated the effects of high- (HF) and low-frequency (LF) transcutaneous electrical nerve stimulation on angiogenesis and myofibroblast proliferation in acute excisional wounds in rat skin. DESIGN: This was an experimental controlled and randomized study. PARTICIPANTS: An excisional wound was made on the back of 90 adult male EPM1-Wistar rats using an 8-mm punch. INTERVENTIONS: The animals were randomly assigned to the HF group (80 Hz), LF group (5 Hz), or control group. Transcutaneous electrical nerve stimulation (pulse duration, 200 microseconds

current amplitude, 15 mA) was delivered (session length, 60 minutes) on 3 consecutive days. MEAN OUTCOME MEASURE: Immunohistochemistry was performed on postoperative days 3, 7, and 14 for counting blood vessels and myofibroblasts. MEAN OUTCOME RESULTS: The LF group had significantly more blood vessels than the HF group on day 3 (P = .004). The HF group had significantly less blood vessels than did the control group on days 7 (P = .002) and 14 (P = .034) and less myofibroblasts than did both the LF and control groups on day 3 (P = .004) and less than did the control group on day 7 (P = .001). CONCLUSION: There seems to be a benefit to the use of LF transcutaneous electrical nerve stimulation in the healing of acute excisional wounds, but further studies are warranted.
Keywords transcutaneous electric nerve stimulation
wound healing
angiogenesis
myofibroblast proliferation
xmlui.dri2xhtml.METS-1.0.item-coverage Philadelphia
Language English
Date 2016
Published in Advances In Skin & Wound Care. Philadelphia, v. 29, n. 8, p. 357-363, 2016.
ISSN 1527-7941 (Sherpa/Romeo, impact factor)
Publisher Lippincott Williams & Wilkins
Extent 357-363
Origin http://dx.doi.org/10.1097/01.ASW.0000488721.83423.f3
Access rights Closed access
Type Article
Web of Science ID WOS:000380631100005
URI https://repositorio.unifesp.br/handle/11600/57492

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