Effects of n-6 and n-3 polyunsaturated acid-rich soybean phosphatidylcholine on membrane lipid profile and cryotolerance of human sperm

Effects of n-6 and n-3 polyunsaturated acid-rich soybean phosphatidylcholine on membrane lipid profile and cryotolerance of human sperm

Author Vireque, Alessandra A. Google Scholar
Tata, Alessandra Google Scholar
Silva, Oswaldo F. L. L. O. Google Scholar
LoTurco, Edson G. Autor UNIFESP Google Scholar
Azzolini, Augusto Autor UNIFESP Google Scholar
Ferreira, Christina R. Google Scholar
Dantas, Marilda H. Y. Google Scholar
Ferriani, Rui A. Google Scholar
Reis, Rosana M. Google Scholar
Abstract Objective: To study the effects of n-6 and n-3 polyunsaturated acid-rich soybean phosphatidylcholine (soy-PC) on sperm cryotolerance with regard to sperm membrane lipid profile, membrane surface integrity, and routine semen parameters. Design: Experimental study. Setting: University-affiliated tertiary hospital. Patient(s): A total of 20 normospermic fertile men. Intervention(s): Semen samples examined for differences in semen parameters, sperm membrane lipid profile, and plasma membrane surface both before and after cryopreservation using basic freezing medium with N-tris(hydroxymethyl)-methyl-2-aminoethane sulfonic acid (TES) and tris-(hydroxymethyl)-aminomethane (TRIS) supplemented with purified soy-PC (TEST-PC) or egg yolk (TEST-Y), both alone or in association (TEST-Y-PC). Main Outcome Measure(s): Conventional semen parameters and membrane lipid profile by matrix-assisted laser/desorption ionization mass spectrometry (MALDI-MS). Result(s): Postthaw sperm cell motility, vitality, and morphology parameters were similar for soy-PC (TEST- PC) and egg yolk ( TEST-Y) cryoprotectants. However, sperm exposed to TEST-Y-PC presented better kinetic parameters, which were similar to the original quality of the fresh semen. Human sperm MALDI-MS lipid profiles revealed that the relative abundance of glycerophospholipids of m/z 760.44 [PC (34:1)+H]+, 781.55 [SM (20:0) +Na]+, 784.55 [PC (36:3)+H]+, 806.64 [PC (38:6)+H]+, 807.64 [SM (22:1)+Na]+, and 809.64 [SM (22:0)+Na]+ increased in soy-PC samples (TEST-PC). Nonetheless, only one lipid (m/z 781.55, [SM (20:0)+Na]+) statistically significantly changed when sperm was cryopreserved in TEST-Y-PC. Conclusion(s): Sphingomyelin was defined as a prospective biomarker of soy-PC treatment, and it could be related to the positive cryoprotective effects of soy-PC in human sperm, opening new perspectives to design of a more efficient synthetic cryoprotectant medium containing purified egg yolk biomolecules combined with soy-PC. (C) 2016 by American Society for Reproductive Medicine.
Keywords L alpha-Phosphatidylcholine
lipid profile
plasma membrane
sperm cryopreservation
xmlui.dri2xhtml.METS-1.0.item-coverage New York
Language English
Sponsor National Institute of Hormones and Woman's Health
National Council for Scientific and Technological Development, CNPq
Date 2016
Published in Fertility And Sterility. New York, v. 106, n. 2, p. 273-U67, 2016.
ISSN 0015-0282 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Inc
Extent 273-U67
Origin http://dx.doi.org/10.1016/j.fertnstert.2016.03.044
Access rights Closed access
Type Article
Web of Science ID WOS:000380763400013
URI https://repositorio.unifesp.br/handle/11600/57482

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