Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele

Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele

Author Carvalho, Natalia S. Autor UNIFESP Google Scholar
Moron, Antonio F. Autor UNIFESP Google Scholar
Menon, Ramkumar Google Scholar
Cavalheiro, Sergio Autor UNIFESP Google Scholar
Barbosa, Mauricio M. Autor UNIFESP Google Scholar
Milani, Herbene J. Autor UNIFESP Google Scholar
Ishigai, Marcia M. Autor UNIFESP Google Scholar
Abstract An increased understanding of the reparative process in fetal membrane following surgical techniques may be helpful to decrease the risks to mother and fetus and avoid adverse pregnancy outcomes. The present study discusses histological evaluation of the fetal membrane following open fetal surgery. Chorioamniotic membranes (n=10) were obtained following birth from pregnancies that underwent open fetal surgery for myelomeningocele. The collagen distribution was quantified using picrosirius-polarization method comparing the suture site with non-suture site. The differences between the collagen fiber percentages at the two sites was evaluated by the paired t-test with P<0.05. The mean gestational age of fetal surgery was 26.09 +/- 0.3 and 33.81 +/- 0.82 weeks at birth. The picrosirius red sign was more intense at the suture site, primarily associated with collagen type 1. Collagen observed in the surgical area was significantly increased (13.22 +/- 2.84%) compared with the non-surgical area (6.16 +/- 1.09%

P<0.0001). It was observed that the reparative activity at the suture site of the fetal membrane was characterized by a significant increase in collagen fibers. The findings suggest nascent collagen synthesis, tissue remodeling and repair of suture site, a mechanism likely to prevent the amniotic fluid leakage and maintain pregnancy following open fetal surgery.
Keywords fetal membrane repair
fetal membrane healing
myelomeningocele
open fetal surgery
spina bifida
xmlui.dri2xhtml.METS-1.0.item-coverage Athens
Language English
Sponsor Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-CAPES, Brazil
Date 2017
Published in Experimental And Therapeutic Medicine. Athens, v. 14, n. 4, p. 3732-3736, 2017.
ISSN 1792-0981 (Sherpa/Romeo, impact factor)
Publisher Spandidos Publ Ltd
Extent 3732-3736
Origin http://dx.doi.org/10.3892/etm.2017.4976
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000410732400143
URI https://repositorio.unifesp.br/handle/11600/57310

Show full item record




File

Name: WOS000410732400143.pdf
Size: 1.103Mb
Format: PDF
Description:
Open file

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account