DNA methylation profile of the DKK2 gene as a biomarker in patients with colorectal cancer

DNA methylation profile of the DKK2 gene as a biomarker in patients with colorectal cancer

Author Silva, T. D. Autor UNIFESP Google Scholar
Felipe, A. V. Autor UNIFESP Google Scholar
Vidigal, V. M. Autor UNIFESP Google Scholar
Saad, S. S. Autor UNIFESP Google Scholar
Forones, N. M. Autor UNIFESP Google Scholar
Abstract Purpose: Epigenetic changes can be detected in precancerous lesions, suggesting that may be involved in the early stages of carcinogenesis. The methylation of specifics genes has been correlated with the outcome of many different types of cancers. This study compared the levels of DNA methylation between normal and tumor tissues from patients with colorectal cancer (CRC). Methods: Candidate genes were screened using the MethylProfiler T PCR Array System and the Dickkopf-related protein 2 (DKK2) genes were selected for study. DNA methylation of these genes was assessed by polymerase chain reaction-high-resolution melting (PCR-HRM) analysis. Results: Out of the 112 patients studied, 68 were controls with a mean age (SD)of 59.9 years (13.4) and 45 were patients with CRC with a mean age (SD) of 64.6 years (13.6). Among the patients with CRC, 25 were women, 28 were diagnosed with colon cancer and 18 were diagnosed with rectal cancer. The number of patients with Stage I, II, III, and IV of the disease, as per the TNM classification, were 2 (4.3%), 20 (43.4%), 18 (39.1%), and 2 (4.3%), respectively. Furthermore, the DKK2 gene had a higher methylation profile in the CRC tissues when compared to normal mucosa (p < 0.001), whereas the methylation profile analysis was not statistically significant when comparing the stages of the disease (p = 0.078). Conclusion: The presence of differentially methylated sequences of the DKK2 gene between the groups showed that the methylation changes of these genes could potentially be prognostic and predictive markers in CRC.
Keywords Biomarkers
Colorectal cancer
DNA methylation
Epigenetics
DKK2 gene
xmlui.dri2xhtml.METS-1.0.item-coverage Ribeirao Preto
Language English
Sponsor Sao Paulo Research Foundation (FAPESP)
Date 2017
Published in Genetics And Molecular Research. Ribeirao Preto, v. 16, n. 4, p. -, 2017.
ISSN 1676-5680 (Sherpa/Romeo, impact factor)
Publisher Funpec-Editora
Extent -
Origin http://dx.doi.org/10.4238/gmr16039816
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000419067100001
URI https://repositorio.unifesp.br/handle/11600/57147

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