Soluble CD40L is associated with increased oxidative burst and neutrophil extracellular trap release in Behcet's disease

Soluble CD40L is associated with increased oxidative burst and neutrophil extracellular trap release in Behcet's disease

Author Perazzio, Sandro Felix Autor UNIFESP Google Scholar
Soeiro-Pereira, Paulo Vitor Google Scholar
dos Santos, Viviane Cardoso Autor UNIFESP Google Scholar
de Brito, Marlon Vilela Autor UNIFESP Google Scholar
Salu, Bruno Autor UNIFESP Google Scholar
Vilela Oliva, Maria Luiza Autor UNIFESP Google Scholar
Stevens, Anne Margherite Google Scholar
Silva de Souza, Alexandre Wagner Autor UNIFESP Google Scholar
Ochs, Hans D. Google Scholar
Torgerson, Troy R. Google Scholar
Condino-Neto, Antonio Google Scholar
Coelho Andrade, Luis Eduardo Autor UNIFESP Google Scholar
Abstract Background: Studies have suggested that soluble factors in plasma from patients with active (aBD) and inactive (iBD) Behcet's disease (BD) stimulate neutrophil function. Soluble CD40 ligand (sCD40L) is an important mediator of inflammation in BD. Its expression and effect on neutrophil oxidative burst and neutrophil extracellular trap (NET) release have not been characterized. In this study, we sought to investigate the role of plasma and the CD40L pathway on NET release and the oxidative burst profile in patients with aBD and iBD. Methods: Neutrophils and peripheral blood mononuclear cells (PBMCs) were obtained from patients with aBD (n = 30), patients with iBD (n = 31), and healthy control subjects (HCs

n = 30). sCD40L plasma concentration was determined in individual samples. A pool of plasma for each group was created. In some experiments, plasma pools were treated with recombinant CD40 (rhCD40-muIg) for sCD40L blockade. NET release and H2O2/O-2-production were determined after stimulation with phorbol 12-myristate 13-acetate, sCD40L, or plasma pool. Flow cytometric analysis was performed to evaluate the expression of (1) CD40, Mac-1, and phosphorylated NF-kappa B p65 on neutrophils and monocytes and (2) CD40L on activated T cells and platelets. CD40L gene expression in PBMCs was determined by qRT-PCR. Results: sCD40L plasma levels were significantly higher in patients with iBD (median 17,234, range 2346-19,279 pg/ml) and patients with aBD (median 18,289, range 413-19,883 pg/ml) than in HCs (median 47.5, range 33.7-26.7 pg/ml

p < 0. 001). NET release was constitutively increased in BD compared with HC. NET release and H2O2/O-2-were higher after stimulation with sCD40L or BD plasma and decreased after sCD40L blockade. Mac-1 expression was constitutively increased in neutrophils of patients with aBD (88.7 +/- 13.2% of cells) and patients with iBD (89.2 +/- 20.1% of cells) compared with HC (27.1 +/- 18.8% of cells

p < 0.01). CD40 expression on phagocytes and CD40L expression on platelets were similar in the three groups. PBMCs as well as nonactivated and activated CD4(+) T cells from patients with BD showed higher CD40L expression. Conclusions: Plasma from patients with aBD exerts a stimulus on NET release and oxidative burst, probably induced by sCD40L.
Keywords Behcet's disease
Neutrophil activation
CD40L pathway
Oxidative burst
Neutrophil extracellular traps
xmlui.dri2xhtml.METS-1.0.item-coverage London
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
Fleury Group
Brazilian National Council for Scientific and Technological Development (CNPq)
Grant number FAPESP: 2011/50292-2
CNPq: 233205/2014-4
CNPq: 476356/2008-3
CNPq: 306902/2013-3
Date 2017
Published in Arthritis Research & Therapy. London, v. 19, p. -, 2017.
ISSN 1478-6354 (Sherpa/Romeo, impact factor)
Publisher Biomed Central Ltd
Extent -
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000413484500002

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