Cutaneous fungal microbiome: Malassezia yeasts in seborrheic dermatitis scalp in a randomized, comparative and therapeutic trial

Cutaneous fungal microbiome: Malassezia yeasts in seborrheic dermatitis scalp in a randomized, comparative and therapeutic trial

Author Kamamoto, C. S. L. Autor UNIFESP Google Scholar
Nishikaku, A. S. Autor UNIFESP Google Scholar
Gompertz, O. F. Autor UNIFESP Google Scholar
Melo, A. S. Autor UNIFESP Google Scholar
Hassun, K. M. Autor UNIFESP Google Scholar
Bagatin, E. Autor UNIFESP Google Scholar
Abstract Malassezia spp in skin microbiome scalp has been implicated in seborrheic dermatitis pathogenesis. Thus, treatment based in antifungal combined to topical keratolitic agents have been indicated as well as oral isotretinoin as it reduces the sebum production, glandular's size and possesses antiinflammatory properties. This randomized, comparative and therapeutic trial aimed toper form the genotypic identification of Malassezia species before and after low-dose oral isotretinoin or topical antifungal treatments for moderate to severe seborrhea and/or seborrheic dermatitis on scalp. Scales and sebum of the scalp were seeded in the middle of modified Dixon and incubated at 32 degrees C. For genotypic identification polymerase chain reaction primers for the ITS and D1/D2 ribossomal DNA were used and followed by samples sequencing. The procedure was conducted before and after therapeutic and randomized intervention for moderate to severe seborrhea/seborrheic dermatitis on the scalp, including oral isotretinoin, 10 mg, every other day and anti-seborrheic shampoo (piroctone olamine), over six months. The M. globosa and M. restricta were the most frequent species isolated on the scalp before and after both treatments. Other non-Malassezia species were also identified. The Malassezia spp. were maintained in the scalp after both treatments that were equally effective for the control of seborrhea/seborrheic dermatitis clinical signs.
Keywords Malassezia
oral isotretinoin
piroctone olamine
polymerase chain reaction
seborrheic dermatitis
xmlui.dri2xhtml.METS-1.0.item-coverage Philadelphia
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (Sao Paulo Research Foundation [FAPESP]), Brazil
Grant number FAPESP: 2010/51049-1
Date 2017
Published in Dermato-Endocrinology. Philadelphia, v. 9, n. 1, p. -, 2017.
ISSN 1938-1972 (Sherpa/Romeo, impact factor)
Publisher Taylor & Francis Inc
Extent -
Origin http://dx.doi.org/10.1080/19381980.2017.1361573
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000428316900001
URI https://repositorio.unifesp.br/handle/11600/57132

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