Activation of the Wnt/beta-catenin pathway in pancreatic beta cells during the compensatory islet hyperplasia in prediabetic mice

Activation of the Wnt/beta-catenin pathway in pancreatic beta cells during the compensatory islet hyperplasia in prediabetic mice

Author Maschio, D. A. Google Scholar
Oliveira, R. B. Google Scholar
Santos, M. R. Google Scholar
Carvalho, Carolina Prado de França Autor UNIFESP Google Scholar
Barbosa-Sampaio, H. C. L. Google Scholar
Collares-Buzato, C. B. Google Scholar
Abstract The Wnt/beta-catenin signaling pathway, also known as the canonical Wnt pathway, plays a role in cell proliferation and differentiation in several tissues/organs. It has been recently described in humans a relationship between type 2 diabetes (T2DM) and mutation in the gene encoding the transcription factor TCF7L2 associated to the Wnt/beta-catenin pathway. In the present study, we demonstrated that hyper plastic pancreatic islets from prediabetic mice fed a high-fat diet (HFD) for 60 d displayed nuclear translocation of active p-catenin associated with significant increases in protein content and gene expression of beta-catenin as well as of cyclins Dl, D2 and c-Myc (target genes of the Wnt pathway) but not of Tcf7I2 (the transcription factor). Meanwhile, these alterations were not observed in pancreatic islets from 30 d HFD-fed mice, that do not display significant beta cell hyperplasia. These data suggest that the Wnt/beta-catenin pathway is activated in pancreatic islets during prediabetes and may play a role in the induction of the compensatory beta cell hyperplasia observed at early phase of T2DM. (C) 2016 Published by Elsevier Inc.
Keywords Wnt/beta-catenin pathway
Prediabetes
Compensatory beta cell hyperplasia
xmlui.dri2xhtml.METS-1.0.item-coverage San Diego
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundo de Apoio ao Ensino, Pesquisa e Extensão (FAEPEX)/UNICAMP
Grant number FAPESP: 2015/25442-1
CNPq: 304991/2015-15
Date 2016
Published in Biochemical and Biophysical Research Communications. San Diego, v. 478, n. 4, p. 1534-1540, 2016.
ISSN 0006-291X (Sherpa/Romeo, impact factor)
Publisher Academic Press Inc Elsevier Science
Extent 1534-1540
Origin https://dx.doi.org/10.1016/j.bbrc.2016.08.146
Access rights Closed access
Type Article
Web of Science ID WOS:000384390200008
URI https://repositorio.unifesp.br/handle/11600/57096

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