Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling

Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling

Author Mimura, Kallyne Kioko Oliveira Autor UNIFESP Google Scholar
Moraes, Andreia R. Google Scholar
Miranda, Aline C. Google Scholar
Greco, Rebecca Google Scholar
Ansari, Tahera Google Scholar
Sibbons, Paul Google Scholar
Greco, Karin V. Google Scholar
Oliani, Sonia Maria Autor UNIFESP Google Scholar
Abstract Biocompatibility of two newly developed porcine skin scaffolds was assessed after 3, 14, 21 and 90 days of implantation in rats. Both scaffolds showed absence of cells, preservation of ECM and mechanical properties comparable to non-decellularised skin before implantation. Host cell infiltration was much prominent on both scaffolds when compared to Permacol (surgical control). At day 3, the grafts were surrounded by polymorphonuclear cells, which were replaced by a notable number of IL-6-positive cells at day 14. Simultaneously, the number of pro-inflammatory M1-macrophage was enhanced. Interestingly, a predominant pro-remodeling M2 response, with newly formed vessels, myofibroblasts activation and a shift on the type of collagen expression was sequentially delayed (around 21 days). The gene expression of some trophic factors involved in tissue remodeling was congruent with the cellular events. Our findings suggested that the responsiveness of macrophages after non-crosslinked skin scaffolds implantation seemed to intimately affect various cell responses and molecular events

and this range of mutually reinforcing actions was predictive of a positive tissue remodeling that was essential for the long-standing success of the implants. Furthermore, our study indicates that non-crosslinked biologic scaffold implantation is biocompatible to the host tissue and somehow underlying molecular events involved in tissue repair.
xmlui.dri2xhtml.METS-1.0.item-coverage London
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2012/21603-2
FAPESP: 2012/13041-4
FAPESP: 2014/18557-4
CNPq: 308144/2014-7
CNPq: 245859/2012-8
Date 2016
Published in Scientific Reports. London, v. 6, p. -, 2016.
ISSN 2045-2322 (Sherpa/Romeo, impact factor)
Publisher Nature Publishing Group
Extent -
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000384923400001

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