Luminescent Ru(II) Phenanthroline Complexes as a Probe for Real-Time Imaging of A beta Self-Aggregation and Therapeutic Applications in Alzheimer's Disease

Luminescent Ru(II) Phenanthroline Complexes as a Probe for Real-Time Imaging of A beta Self-Aggregation and Therapeutic Applications in Alzheimer's Disease

Author Silva, Debora E. S. Google Scholar
Cali, Mariana P. Google Scholar
Pazin, Wallance M. Google Scholar
Carlos-Lima, Estevão Autor UNIFESP Google Scholar
Salles Trevisan, Maria Teresa Google Scholar
Venancio, Tiago Google Scholar
Arcisio-Miranda, Manoel Autor UNIFESP Google Scholar
Ito, Amando S. Google Scholar
Carlos, Rose M. Google Scholar
Abstract The complexes, cis-[Ru(phen)(2)(Apy)(2)](2+), Apy = 4-aminopyridine and 3,4-aminopyridine, are stable in aqueous solution with strong visible absorption. They present emission in the visible region with long lifetime that accumulates in the cytoplasm of Neuro2A cell line without appreciable cytotoxicity. The complexes also serve as mixed-type reversible inhibitors of human AChE and BuChE with high active Site contact. cis-[Ru(phen)(2)(3,4Apy)(2)](2+) competes efficiently with DMPO by the OH center dot radical. Luminescence using fluorescence lifetime imaging (FLIM) enables real-time imaging of the onformational Changes of the self-aggregation, of A beta with incubation of complexes (0-24 h) in phosphate buffer at micromolar concentrations. By this technique, we identified protofibrillsin the self-assembly of A beta(1-40) and globular structures in the short fragment A beta(15-21) in aqueous solution.
xmlui.dri2xhtml.METS-1.0.item-coverage Washington
Language English
Sponsor FAPESP [2014/12538-8, 2014/07935-8, 2012/02065-0, 2014/24643-0, 2014/26895-7]
CNPq [304981/2012-5]
CAPES
Grant number FAPESP: 2014/12538-8
FAPESP: 2014/07935-8
FAPESP: 2012/02065-0
FAPESP: 2014/24643-0
FAPESP: 2014/26895-7
CNPq: 304981/2012-5
Date 2016
Published in Journal Of Medicinal Chemistry. Washington, v. 59, n. 19, p. 9215-9227, 2016.
ISSN 0022-2623 (Sherpa/Romeo, impact factor)
Publisher Amer Chemical Soc
Extent 9215-9227
Origin http://dx.doi.org/10.1021/acs.jmedchem.6b01130
Access rights Closed access
Type Article
Web of Science ID WOS:000385607100034
URI https://repositorio.unifesp.br/handle/11600/56902

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