Preoperative vitamin C supplementation improves colorectal anastomotic healing and biochemical parameters in malnourished rats

Preoperative vitamin C supplementation improves colorectal anastomotic healing and biochemical parameters in malnourished rats

Author Danielski, Lucineia Gainski Google Scholar
Walczewski, Eduardo Google Scholar
de Jesus, Clovisa Reck Google Scholar
Florentino, Drielly Google Scholar
Della Giustina, Amanda Google Scholar
Goldim, Mariana Pereira Google Scholar
Kanis, Luiz Alberto Google Scholar
Pereira, Gregório Wrublevski Autor UNIFESP Google Scholar
Pereira, Volnei D. Google Scholar
Felisberto, Francine Google Scholar
Petronilho, Fabricia Google Scholar
Abstract The objective of this study was to evaluate the effect of supplementation with vitamin C on intestinal anastomosis healing in malnourished rats. Male Wistar rats were divided into three groups: (1) sham, well-nourished rats that received vehicle

(2) FR+Veh, rats that were subjected to food restriction and received vehicle

and (3) FR+VC, rats that were subjected to food restriction and received vitamin C. Four days before surgery, the animals received vitamin C (100 mg/kg/day) via gavage and underwent colon resection with anastomosis in a single plane. The survival rate of rats was monitored until day 7 after surgery. Regarding anastomosis tissues, we examined intra-abdominal adhesion index, hydroxyproline content, collagen density, inflammatory parameters, and oxidative damage to proteins and lipids. Malnutrition decreases body weight and increases mortality

the survival rate was 90 % in group 1, 60 % in group 2, and 80 % in group 3. Vitamin C was able to increase hydroxyproline concentration and density of collagen and decrease the intra-abdominal adhesion index, as well as the infiltration of neutrophils and oxidative damage to proteins in malnourished rats compared to group treated with vehicle. Preoperative vitamin C supplementation can improve the intestinal anastomosis healing, biochemical alterations, and prolong survival in rats subjected to food restriction.
Keywords Inflammation
Oxidative stress
Intestinal anastomosis
Vitamin C
Malnutrition
xmlui.dri2xhtml.METS-1.0.item-coverage New York
Language English
Date 2016
Published in International Journal Of Colorectal Disease. New York, v. 31, n. 11, p. 1759-1766, 2016.
ISSN 0179-1958 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 1759-1766
Origin http://dx.doi.org/10.1007/s00384-016-2647-x
Access rights Closed access
Type Article
Web of Science ID WOS:000385197300003
URI https://repositorio.unifesp.br/handle/11600/56872

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