Analysis of the functional aspects and seminal plasma proteomic profile of sperm from smokers

Analysis of the functional aspects and seminal plasma proteomic profile of sperm from smokers

Author Antoniassi, Mariana Pereira Autor UNIFESP Google Scholar
Intasqui, Paula Autor UNIFESP Google Scholar
Camargo, Mariana Autor UNIFESP Google Scholar
Zylbersztejn, Daniel Suslik Autor UNIFESP Google Scholar
Carvalho, Valdemir Melechco Google Scholar
Cardozo, Karina H. M. Google Scholar
Bertolla, Ricardo Pimenta Autor UNIFESP Google Scholar
Abstract Objective To evaluate the effect of smoking on sperm functional quality and seminal plasma proteomic profile. Patients and Methods Sperm functional tests were performed in 20 non-smoking men with normal semen quality, according to the World Health Organization (2010) and in 20 smoking patients. These included: evaluation of DNA fragmentation by alkaline Comet assay

analysis of mitochondrial activity using DAB staining

and acrosomal integrity evaluation by PNA binding. The remaining semen was centrifuged and seminal plasma was used for proteomic analysis (liquid chromatographytandem mass spectrometry). The quantified proteins were used for Venn diagram construction in Cytoscape 3.2.1 software, using the PINA4MS plug-in. Then, differentially expressed proteins were used for functional enrichment analysis of Gene Ontology categories, Kyoto Encyclopedia of Genes and Genomes and Reactome, using Cytoscape software and the ClueGO 2.2.0 plug-in. Results Smokers had a higher percentage of sperm DNA damage (Comet classes III and IV

P < 0.01), partially and fully inactive mitochondria (DAB classes III and IV

P = 0.001 and P = 0.006, respectively) and non-intact acrosomes (P < 0.01) when compared with the control group. With respect to proteomic analysis, 422 proteins were identified and quantified, of which one protein was absent, 27 proteins were under-represented and six proteins were over-represented in smokers. Functional enrichment analysis showed the enrichment of antigen processing and presentation, positive regulation of prostaglandin secretion involved in immune response, protein kinase A signalling and arachidonic acid secretion, complement activation, regulation of the cytokinemediated signalling pathway and regulation of acute inflammatory response in the study group (smokers). Conclusion In conclusion, cigarette smoking was associated with an inflammatory state in the accessory glands and in the testis, as shown by enriched proteomic pathways. This state causes an alteration in sperm functional quality, which is characterized by decreased acrosome integrity and mitochondrial activity, as well as by increased nuclear DNA fragmentation.
Keywords acrosome
DNA damage
seminal plasma
xmlui.dri2xhtml.METS-1.0.item-coverage Hoboken
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fleury Projects Funding (Fleury Group)
Grant number CNPq: 160857/2012-0
CNPq: 306616/2013-0
Date 2016
Published in Bju International. Hoboken, v. 118, n. 5, p. 814-822, 2016.
ISSN 1464-4096 (Sherpa/Romeo, impact factor)
Publisher Wiley
Extent 814-822
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000386468600025

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